摘要
目的探讨微RNAlet-7a-3基因启动子甲基化与糖尿病肾病的关系。方法采用生物信息学方法预测let-7a-3基因启动子区域甲基化位点,采用real-time PCR及甲基化特异性PCR检测20例糖尿病肾病(DN)患者、20例糖尿病患者(DM)以及20例正常对照(CON)的let-7a-3表达水平及启动子区域甲基化水平。结果 let-7a-3在DN组呈低表达。同时,DN组的平均甲基化率为96.2%±6.2%,DM组的平均甲基化率为76.6%±18.9%,正常组的平均甲基化率为63.2%±28.4%。DN组的平均甲基化水平较DM组和正常组显著增高(P<0.01)。结论 let-7a-3启动子区域高甲基化可能是糖尿病肾病患者let-7a-3低表达的一个重要因素,并参与调控糖尿病肾病的发生与发展。
Objective To explore the relationship between microRNA let-7a-3 gene promoter methylation and diabetic nephropathy（ DN）. Methods Methylation sites were predicted in the promoter region of let-7a-3 gene by bioinformatics methods. Real-time PCR and real-time methylation specific PCR（ q MSP） were used to detect the expression and methylation level in 20 cases of diabetic nephropathy patients（ DN group）,20 patients with diabetes mellitus（ DM group） and 20 cases of normal control（ CON group）. Results let-7a-3 was down-expressed in DN group as compared with that in DM and CON groups. Moreover,the average methylation ratio of let-7a-3 was96. 2% ± 6. 2% in DN group,76. 6% ± 18. 9% in DM group,and 63. 2% ± 28. 4% in CON group. The average methylation ratio of let-7a-3 in DN group was significantly higher than that in CON and DM groups（ P〈0. 01）.Conclusions Promoter hypermethylation of let-7a-3 may play a role in the down-expression of let-7a-3,and may participate in the regulation of the occurrence and development of diabetic nephropathy.
出处
《基础医学与临床》
CSCD
2016年第4期474-479,共6页
Basic and Clinical Medicine
基金
国家自然科学基金(81270912)
重庆市科委一般项目(cstc2013jcyj A10044)
