与小鼠小脑发育相关的一种长非编码RNA Gm2694的鉴定

Characterization of a novel lncRNA Gm2694 specifically expressed in mice cerebellum

目的鉴定与小鼠小脑发育相关的长非编码RNA,并验证其各剪接变异体的存在。方法用芯片技术分析小鼠小脑发育不同阶段RNA的表达差异;用实时定量PCR技术对芯片结果加以验证;针对目的 RNA,验证其各剪接变异体的存在及在小鼠小脑中的表达量。结果 1)长非编码RNA Gm2694在小鼠小脑发育过程中呈动态变化趋势;2)其在小鼠小脑中特异性表达;3)在小鼠小脑中检测到Gm2694有8种表达水平各不相同的剪接变异体,其中除Ensembl数据库预测的7个之外,发现了1个新的表达于小脑的剪接变异体。结论 Gm2694是一种在小鼠小脑特异性表达的长非编码RNA,存在8种表达量各不相同的剪接变异体。

Objective To identify novel lncRNAs involved in cerebellar neurogenesis and validate the expression of related splice variants. Methods Microarray analysis of infant（ P7） and adult（ P28） mice cerebellar RNA was performed to discover novel lncRNAs involved in mice cerebellum neurogenesis. RT-q PCR was then performed to validate the expression of candidate lncRNAs and splice variants. Results A novel lncRNA,Gm2694,was found to be specifically expressed in mice cerebellum in a developmentally regulated manner. In total eight kinds of splice variants has been detected in cerebellum with various expression levels： besides 7 variants as predicted in the Ensembl databases,another novel variant was identified in mice cerebellum by RACE c DNA amplification. Conclusions Temporal spatial analysis reveals that Gm2694 is a novel lncRNA possibly involved in mice cerebellum neurogenesis.