靶向ANGPTL4基因shRNA慢病毒载体抑制SiHa细胞迁移与侵袭

The lentivirus-mediated shRNA targeting ANGPTL4 inhibits the migration and invasion of SiHa cells

目的探讨慢病毒介导短发夹RNA(shRNA)沉默血管生成素样蛋白4(ANGPTL4)基因对人宫颈癌细胞系Si Ha细胞迁移与侵袭的影响。方法实验分为3组,即空白对照组、阴性对照组及LV3/ANGPTL4组。用ANGPTL4shRNA慢病毒干扰载体感染Si Ha细胞,Western blot检测Integrinβ1、VEGF及MMP9蛋白表达,划痕实验检测Si Ha细胞迁移能力,Transwell实验检测Si Ha细胞迁移与侵袭能力。结果重组慢病毒载体成功感染Si Ha细胞,与空白对照组及阴性对照组比较,LV3/ANGPTL4组Si Ha细胞Integrinβ1、VEGF及MMP9蛋白表达水平降低(P<0.01),细胞迁移与侵袭能力减弱(P<0.05)。结论靶向ANGPTL4基因shRNA慢病毒载体可降低Si Ha细胞Integrinβ1、VEGF及MMP9蛋白表达水平,抑制Si Ha细胞迁移与侵袭能力。

Objective To investigate the effect of lentivirus-mediated shRNA silencing angiopoietin-like protein 4（ ANGPTL4） gene on migration and invasion of cervical cancer cell line Si Ha. Methods In this experiment,three groups were designed： control,negative control and LV3 / ANGPTL4 groups. The shRNA for lentiviral interference vectors were infected into Si Ha cells. The expressions of Integrinβ1,VEGF and MMP9 protein were detected by Western blot. The migration and invasion ability of Si Ha cells were detected by scratch wound assay and Transwell respectively. Results ANGPTL4 shRNA lentiviral interference vectors were successfully infected into Si Ha cells.Compared with those in control group and negative control group,the expressions of Integrinβ1,VEGF and MMP9 protein in LV3 / ANGPTL4 group were down-regulated（ P〈0. 01）. The migration and invasion ability of Si Ha cells in LV3 / ANGPTL4 group were significantly decreased（ P〈0. 05）. Conclusions Lentiviral-shRNA vector targeting at ANGPTL4 gene can down-regulate the expression of Integrinβ1,VEGF and MMP9 protein in Si Ha cells. It can also effectively inhibit the migration and invasion of Siha cells.