转移生长因子基因单核苷酸多态性与肺癌易感性的关系研究

Relationship between transforming growth factor gene single nucleotide polymorphisms and susceptibility of lung cancer

目的研究转移生长因子（TGF）基因上rsl800469（TGFl-509C／T）和rsl982073（TGFl＋869T／C）位点基因单核苷酸多态性与肺癌易感性之间的关系。方法选择原发性肺癌患者193例（病例组）和211例同期就诊的非肿瘤患者（对照组）。应用实时荧光定量逆转录聚合酶链反应方法进行TGF基因单核苷酸多态性的基因分型，比较两组人口学特征、相关危险因素暴露及基因单核苷酸多态性的差异。结果对照组在TGFl＋869T／C和TGFl-509C／T的基因型分布频率经拟合优度r检验，符合Hardy-Weinberg遗传平衡定律（P〉0．05）。TGFl＋869T／C的基因型TT、TC、CC分布频率在病例组分别是16．1％（31／193）、55．4％（107／193）、28．5％（55／193），在对照组的分布频率分别是22．3％（47／211）、50．2％（106／211）、27．5％（58／211），差异无统计学意义（P〉0．05）。TGFl-509C／T的基因型TT、TC、CC分布频率在病例组分别是28．0％（54／193）、56．0％（108／193）、16．1％（31／193），在对照组的分布频率分别是27．0％（57／211）、51．2％（108／211）、21．8％（46／211），差异无统计学意义（P〉0．05）。T等位基因携带者患肺癌的风险与c等位基因携带者患肺癌的风险相比差异无统计学意义（P〉0．05）。结论北方汉族人群中TGF基因单核苷酸多态性与肺癌易感性无关。

Objective To investigate the association of transforming growth factor （TGF） gene rs1800469 （TGF1-509C/T） and rs1982073 （TGF1 ＋869T/C） single nucleotide polymorphisms （SNP） with susceptibility of lung cancer. Methods The case group consisted of 193 histologically diagnosed lung cancer patients, and control group of 211 controls were selected from cancer-free patients at the same time. The single nucleotide polymorphisms of rs1800469 （TGF1-509C/T） and rs1982073（TGFl＋ 869T/C）were analyzed by real-time fluorescent quantitative reverse transcription polymerase chain reaction method. The demographic characteristics, correlated risk factors of exposure and SNP between 2 groups were compared. Results The genotype distribution frequency of TGF1 ＋ 869T/C and TGF1-509C/T in control group was accorded with Hardy-Weinberg law （P〉0.05）. The TT, TC and CC genotype distribution frequency in TGFl＋869T/C in case group were 16.1% （31/193）, 55.4% （107/193） and 28.5% （55/193）, while these in control group were 22.3% （47/211）, 50.2% （106/211） and 27.5% （58/211）, and there were no statistical differences （P 〉 0.05）. The TT, TC and CC genotype distribution frequency in TGF1-509C/T in case group were 28.0% （54/193）, 56.0% （108/193） and 16.1% （31/193）, while these in control group were 27.0% （57/211）, 51.2% （108/211） and 21.8% （46/211）, and there were no statistical differences （P 〉 0.05）. The allele in rs1800469 （TGF1-509C/T） and rs1982073 （TGF1 ＋869T/C） showed no association with lung cancer （P 〉 0.05）. Conclusions TGF gene SNP may not be associated with susceptibility to lung cancer in Hart Chinese in North China.