当归提取物对慢性不可预知性应激抑郁模型大鼠抑郁行为及脑内炎症因子的影响

Effect of Angelica Sinensis extract on depression behaviors and brain inflammatory factors in depres-sion model rats induced by chronic unpredictable mild stress

目的：观察当归提取物对慢性不可预知性应激抑郁模型大鼠抑郁行为及海马和前额叶皮质炎症因子（ TNF-α，IL-6）的影响。方法成年雄性SD大鼠32只，体质量140～160 g，随机分为四组：正常组、应激组、应激＋氟西汀组、应激＋当归组。应激、应激＋氟西汀和应激＋当归这三组大鼠给予为期5周的慢性不可预知性应激刺激，给药组在最后3周每天分别给予当归、氟西汀药物灌胃治疗。给药结束后进行糖水偏爱、强迫游泳和旷场实验检测。行为学检测结束后第2天给予腹腔注射水合氯醛（350 mg／kg）麻醉，直接断头取大鼠海马和前额叶皮质进行ELISA检测TNF-α、IL-6表达水平。结果与正常组相比，应激组大鼠糖水摄入比率[（42±15）％]明显减少、漂浮时间[（68．28±16．50）s]明显延长以及穿越格子数[（31．25±21．56）次]明显减少（P＜0．01），海马炎症因子TNF-α[（206．14±30．53）pg／ml]、IL-6[（369．51±103．81）pg／ml]和前额叶皮质区炎症因子TNF-α[（199．33±25．67）pg／ml]、IL-6[（347．74±81．04）pg／ml]表达明显增加（P＜0．01）。与应激组相比，当归组大鼠糖水摄入比率（0．66±0．21）明显增加、漂浮时间[（32．53±10．26）s]显著缩短以及穿越格子数[（83．00±23．25）次]增加（P＜0．01），海马炎症因子TNF-α[（53．42±12．43）pg／ml]、IL-6[（93．84±13．19）pg／ml]和前额叶皮质炎症因子TNF-α[（57．58±8．33）pg／ml]、IL-6[（91．18±17．37）pg／ml]表达明显下降（P＜0．01）。结论当归提取物可能通过调控海马和前额叶皮质区炎症因子（ TNF-α、IL-6）水平表达来改善慢性不可预知性应激大鼠抑郁症状。

Objective To observe the effects of Radix Angelica Sinensis （ RAS） on depression be-havior and cytokines,TNF-αand IL-6,in the hippocampus and prefrontal cortex of depression model rats in-duced by chronic unpredictable mild stress （ CUMS） .Methods 32 adult male rats weighting 140-160 g were randomly divided into 4 groups： Control,CUMS,CUMS＋fluoxetine and CUMS＋RAS groups.CUMS procedure went on 5 consecutive weeks and during the last 3 weeks the rats in CUMS＋fluoxetine and CUMS＋RAS groups were taken RAS or fluoxetine via intragastric administration.After 5-week CUMS procedure, rats were subjected to sucrose preference test,forced swimming test and open field test.After behavioral tests were finished,all rats were anesthetized with 10%chloral hydrate （350 mg/kg,intraperitoneal injection） and then decapitated.The hippocampus and prefrontal cortex of rats were separated and ELISA was used to detect the expression of TNF-αand IL-6 in these regions.Results Comparing with control rats, rats exposed to CUMS showed decreased sucrose preference ratio（（42±15）%）,prolonged immobility time （（68.28±16.50） s）,decreased crossing numbers （31.25±21.56） and increased TNF-α（（206.14±30.53）pg/ml） and IL-6 （（369.51±103.81）pg/ml） expression in hippocampus and TNF-α（（199.33±25.67）pg/ml） and IL-6 （（347.74±81.04） pg/ml） expression in prefrontal cortex （ P〈0.01）.However,RAS treated rats reversed the behavioral changes such as sucrose preference ratio（（66±21）%）,immobility time （（32.53±10.26）s） and crossing numbers （ 83.00 ±23.25 ） , meanwhile reduced TNF-α（ （ 53.42 ±12.43 ） pg/ml ） and IL-6 （（93.84±13.19）pg/ml） expression in hippocampus,TNF-α（（57.58±8.33）pg/ml） and IL-6（（91.18±＆amp;nbsp;17.37） pg/ml） expression in prefrontal cortex among stress rats （ P〈0.01） .Conclusion RAS can amelio-rate CUMS induced depression behaviors of rats through regulating hippocampus and prefrontal cortex cyto-kines （ TNF-α,IL-6） .