肠吉泰对肠易激综合征内脏高敏感大鼠TRPV_1表达的影响

Effect of Chang Ji Tai on TRPV_1 in the Rats with Visceral Hypersensitivity

目的:探讨中药制剂肠吉泰对内脏高敏感性模型大鼠的调节作用。方法:选择新生SD大鼠随机分为空白组、模型组、阳性药物Capsazepine组及肠吉泰低、中、高剂量组,每组10只。采用Al-chaer直肠醋酸刺激法建立内脏高敏感性大鼠模型。造模期间阳性药物组给予腹腔注射瞬时感受器电位香草素受体-1(TRPV1)阻断剂Capsazepine,其余各组(除空白组外)均给予相同体积的溶剂腹腔注射;造模结束2周后,肠吉泰各剂量组每日分别给予相应剂量的中药浸膏灌胃,空白组、模型组和阳性药物组分别给予等体积去离子水灌胃。干预4周后,大鼠腹外斜肌埋植电极,外接Power Lab记录仪,采用结直肠气囊扩张法记录大鼠腹外斜肌电压变化以评估内脏敏感性;使用Real-time PCR法检测下丘脑、背根神经节和结肠组织TRPV1mRNA含量。结果:当气囊压力在60 mm Hg时,模型组大鼠腹外斜肌电压值较空白组明显升高(P<0.05),肠吉泰高剂量组的电压较模型组明显降低(P<0.01)。模型组大鼠下丘脑、背根神经节和结肠组织的TRPV1mRNA表达较空白组均明显增高(P<0.05);肠吉泰中、高剂量组背根神经节与下丘脑的TRPV1mRNA表达,以及肠吉泰各剂量组结肠的TRPV1mRNA表达均明显低于模型组(P<0.05或P<0.01)。结论:肠吉泰可降低内脏敏感性,其作用可能与降低下丘脑、结肠和背根神经节TRPV1mRNA表达有关。

Objective： To observe the effect of Chang Ji Tai（ CJT） on TRPV1 in Hypothalamus,dorsal root ganglia（ DRG） and colon of the rats with visceral hypersensitivity. Methods： Neonatal Sprague-Dawley rats were randomly divided into 6 groups： normal control group,model control group,positive control group,CJT low dose group,CJT middle dose group and CJT high dose group,10 in each group. Visceral hypersensitivity model was induced by intrarectal acetic acid irritation in the latter 5 groups. During models making period,Capsazepine was intraperitoneal injected in positive control group while the other groups except blank group were injected the same quantity solvent through abdominal cavity; two weeks after the models making,CJT each dose group was given intragastric administration with Chinese medical concrete,and blank group,model group and positive control group were given intragastric administration with equivalent deionized water.. After 4 weeks treatment,rectal balloon distension was applied to evaluate visceral hypersensitivity by measuring electrical discharge of external oblique. Hypothalamus,L5 ~ S1 DRG and 1cm descending colon tissue were isolated for TRPV1 mRNA measurement respectively by real-time polymerase chain reaction（ RT-PCR）. Results： Compared to the normal control group,the 60 mm Hg pressure inducing electrical discharge of external oblique was significantly higher in model control group（ P 0. 05）,while compared to the model control group,it＇s significantly lower in CJT high dose group（ P 0. 01） with the same pressure.Compared to the normal control group,the Hypothalamus,DRG and colon TRPV1 levels were significantly higher in model control group（ P 0. 05）. TRPV1 levels in many CJT treatment groups were significantly lower than model control group（ P 0. 05 or P 0. 01）. Conclusion： CJT could reduce the visceral hyper sensitivity,and the function mechanism maybe has the relationship with decreasing the levels of Hypothalamus,DRG and colon TRPV1 mRNA.