夏枯草口服液对雌激素受体阳性乳腺癌细胞凋亡的影响

Apoptotic Effect of the Extract of Xiakucao Oral Solution on Estrogen Receptor-positive Human Breast Cancer Cells

目的:研究夏枯草口服液对雌激素受体(ER)阳性人乳腺癌细胞凋亡的影响。方法:将ER阳性型人乳腺癌细胞系MCF-7及ER阴性型人乳腺癌细胞系MDA-MB-231、SK-BR-3分别与不同浓度(0、25、50、100 mg/ml)的夏枯草口服液提取物共孵育。采用噻唑蓝(MTT)法检测细胞存活率;流式细胞术及Annexin V/PI双染色检测细胞周期和细胞凋亡;ELISA法检测雌激素应答基因表达量;Western blot法检测ERα蛋白表达情况。结果:1夏枯草口服液提取物呈剂量依赖性地抑制MCF-7细胞的生长,但对MDAMB-231、SK-BR-3细胞无显著作用。2夏枯草口服液提取物可诱导MCF-7细胞产生凋亡,在mRNA水平降低MCF-7细胞内雌激素应答基因的表达,并降低ERα蛋白表达。结论:夏枯草口服液可诱导ER阳性乳腺癌细胞的凋亡,用于治疗ER阳性乳腺癌。

Objective： To study the potential apoptotic effect of Xiakucao oral solution,a patent traditional Chinese medicine,on estrogen receptor（ ER） positive human breast cancer cells. Methods： The extract of Xiakucao oral solution（ EPV） was treated in ER-positive type human breast cancer cell line MCF-7 and ER-negative type human breast cancer cell line MDA-MB-231,SK-BR-3,respectively. Relative cell viabilities were determined by the MTT method; apoptosis and cell cycle were analyzed by Annexin V / PI dual staining with flow cytometry; the protein expression levels of ERα were detected by Western blot. Gene expression levels were analyzed using ELISA assay kit. Results： 1 EPV reduced the viability of MCF-7cells dose-dependently. However,EPV did not show significantly inhibitory effect on the viabilities of MDA-MB-231 and SKBR-3 cells. 2 EPV induced apoptosis in MCF-7 cells. EPV reduced the level of mRNA within the MCF-7 cell luciferase activity and estrogen-responsive gene expression,and a dose-dependent decrease ERα. Conclusion： EPV could induce apoptosis in ER-positive breast cancer cells,which provide novel information for clinical application of xiakucao oral solution for the ER-positive breast cancer patients.