低强度循环载荷训练对髌骨髌腱结合部损伤和髌骨微细结构的影响

The Effect of Low Intensity Cyclical Load on the Patella- patella Tendon Junction Injury and Patella Microstructure

目的:本研究利用循环载荷动物模型,比较休息和低强度运动对髌骨髌腱结合部(patella-patella tendon junction,PPTJ)损伤及对髌骨的影响。实验方法:12只成年雌性新西兰大白兔,随机筛选分组分为4周循环载荷组(LD-RST,n=6),8周循环载荷组(LD-LI,n=6),左后肢进行实验,右后肢为空白对照。实验过程中,前4周两组实验动物统一接受强度为7.17N/S共24小时的循环载荷。在实验的第4周后,LD-RST组停止实验,LD-LI组将强度改为4.2N/共24小时的循环载荷。8周后所有动物统一进行取材,将取材后组织进行Micro-CT进行并进行H&E染色,使用图像采集系统进行组织学分析。结果:H&E染色测量纤维软骨带厚度LD-LI组和LD-RST组没有显著差异,LD-LI组的胶原排列比LD-RST组更加紧密整齐。骨量和骨小梁结果显示实验组和对照组没有显著差异。结论:4周休息不能促进髌腱结合部损伤的恢复。4周低强度的循环载荷没有加重损伤和髌骨增生,并且对肌腱的重建修复具有促进作用。

Objective： This study was to compare the effects of rest and low intensity exercise on tendon patella- patella junction（ PPTJ） injury and patella with a cyclic loading animal injury model. Experiment design： 12 adult female New Zealand white rabbits were randomly assigned to a four- week cyclical load group（ LD- RST,n =6） and a 8- week cyclical load group（ LD- LI,n = 6）. During the experiment in the first 4 weeks,the two groups of rabbits received cyclical load of 7. 17 N / S·24 hours. After four weeks,the experiment on the LD-RST group was stopped and the load on the LD- LI group was changed into 4. 2N / S·24 hours. After 8 weeks,tissues were collected from all the rabbits. The collected tissues were first scanned by Micro- CT and treated by HE staining and then used for histological analysis by the image acquisition system. Results： There was no significant difference between the LD- LI group and the LD- RST group in the thickness of the fibrocartilage by HE staining,and the collagen array in the LD- LI group is tighter and more regular than the LD- RST group. There is no significant difference in patellar microstructure between the experimental group and the control group. Conclusion： 4 weeks of rest can not promote the recovery of bone tendon junction injury. 4 weeks of low intensity of cyclic loading did not aggravate the damage and patella hyperplasia,and can promote the tendon reconstruction and repair.