冬病夏治穴位贴敷疗法对慢性支气管炎缓解期患者炎症细胞因子及血清肺表面活性蛋白的影响

Effect of the Method Winter Diseases Treated in Summer Using Point Application Therapy on Inflammatory Cytokines and Surfactant Pulmonary Protein in Patients with Chronic Bronchitis During Alleviation

目的探讨冬病夏治穴位贴敷疗法治疗慢性支气管炎缓解期的可能作用机制。方法选取慢性支气管炎缓解期患者50例为治疗组,50例健康志愿者为对照组。治疗组在头伏、二伏、三伏节气的第1天分别进行穴位贴敷治疗,共3次,对照组不进行任何干预。比较治疗前后两组肿瘤坏死因子α(TNF-α)、γ干扰素(IFN-γ)、白细胞介素4(IL-4)、白细胞介素8(IL-8)、白细胞介素10(IL-10)、血清肺表面活性蛋白A(SP-A)、血清肺表面活性蛋白D(SP-D)水平。结果与对照组比较,治疗组治疗前血清IL-4、IL-8、TNF-α水平升高,血清IL-10、IFN-γ、SP-A、SP-D水平降低(P<0.05)。治疗组治疗后血清IL-4、IL-8水平较治疗前降低,血清IL-10、IFN-γ、SP-A、SP-D水平较治疗前升高(P<0.05);与对照组比较,治疗后治疗组血清IL-4、TNF-α水平升高,IFN-γ、IL-10、SP-A、SP-D水平降低(P<0.05),IL-8差异无统计学意义(P>0.05)。结论冬病夏治穴位贴敷法能调节炎症细胞因子的释放,降低炎症发生的程度,可能是其预防慢性支气管炎复发的机制之一。

Objective To explore the possible mechanism in treating chronic bronchitis alleviation with curing winter diseases in summer using point application therapy. Methods Fifty patients with chronic bronchitis during alleviation were assigned as the treatment group,and 50 healthy volunteers as the control group. The treatment group received point application on the first day of the first,the second,and the third dogdays,respectively,for 3 times in total. The control group was not intervened. Serum levels of tumor necrosis factor α（ TNF-α）,interferon γ（ IFN-γ）,interleukin 4（ IL-4）,interleukin 8（ IL-8）,interleukin 10（ IL-10）,surfactant pulmonary protein A（ SP-A）,and surfactant pulmonary protein D（ SP-D） of two groups were compared before and after treatment. Results Compared with those in the control group,serum levels of IL-4,IL-8 and TNF-α in the treatment group increased while levels of serum IL-10,IFN-γ,SP-A and SP-D decreased before treatment（ P〈0. 05）. Serum levels of IL-4and IL-8 decreased while serum levels of IL-10,IFN-γ,SP-A and SP-D increased in the treatment group after treatment compared with before（ P〈0. 05）. Compared with those in the control group,serum levels of IL-4 and TNF-αin the treatment group increased while serum levels of IL-10,IFN-γ,SP-A and SP-D decreased after treatment（ P〈0. 05）. There was no statistical significant difference in level of IL-8 between two groups. Conclusion The method winter diseases treated in summer using point application therapy could regulate the release of inflammatory cytokines and decrease the degree of inflammation occurrence,which may be one of the mechanisms in preventing recurrence of chronic bronchitis.