秋葵中总黄酮提取工艺探讨

Discussion of extraction technology of flavonoids from okra

分别用超声微波联用法与纤维素酶浸提法2种方法提取秋葵中黄酮类化合物,用硝酸铝-亚硝酸钠比色法测定提取的总黄酮含量,采用正交试验对2种方法下提取的总黄酮得率进行数据分析与比较。结果表明,纤维素酶浸提法比超声微波联用法提取秋葵黄酮得率高,纤维素酶浸提法最佳工艺:加酶量1.5%,酶解时间2 h,酶解温度50℃,酶解p H4.8,总黄酮得率为4.843%;超声微波联用法最佳工艺:微波功率550 W,料液比1:50(g/m L),超声时间30 min,微波时间60 s,总黄酮得率为3.278%,纤维素酶浸提法比超声微波联用法的黄酮得率提高了1.565%。

Ultrasonic-microwave synergistic extraction technique and cellulase extraction technique were employed to extract the flavonoids from okra respectively.The Aluminum nitrate-sodium nitrite colorimetry were used to deter mine the total flavonoids content.Making data analysis and comparisons of the yield of two extraction method by orthogonal experiment design.The results showed that the flavonoid yield under the method of cellulase extraction technique was a little bit higher than the ultrasonic-microwave synergistic extraction technique.The optimal process of the cellulase extraction technique as follows：cellulose amount was 1.5%,the enzymatic hydrolysis time was 2 h,the extraction temperature was 50 ℃,p H was 4.8,the yield of the total flavonoids was 4.843%.The optimal process of the Ultrasonic-microwave synergistic extraction technique as follows：microwave power was 550 W,solid-liquid ratio was 1：50（g/m L）,ultrasonic treatment time was 30 min,microwave extraction time was 60 s,the yield of total flavonoids was 3.278%,comparing to the utrasonic-microwave synergistic extraction technique,the yield of the flavonoids by the cellulose extraction technique was increased by 1.565%.