一种保护黄粉虫蛋白质的油脂提取法

An oil extraction method of protecting Tenebrio molitor protein

黄粉虫幼虫具有丰富的营养成分,为综合利用,提高其附加价值,采用2步骤处理法。首先对黄粉虫进行预处理,确定水分标准4%以下,其次采用不同温度不同方式进行温和的索式提取法抽取油脂以及抽取率的方差分析,并用凯氏定氮法和考马斯亮蓝法(BBC G-250)测定其脱脂前后蛋白含量。结果表明:综合考虑保护蛋白质并兼顾油脂的最佳组合为第一次去水分的最佳温度为65℃,第二次提取的最佳温度为45℃,其油脂提取率达到19.99%,萃取率达到16.12%,蛋白质提取率3.68%,其质量浓度为0.3032 mg/m L。

Tenebrio molitor larvae has rich nutrition,in order to the comprehensive utilization,and improving the additional value,so using two step processes in this paper.Firstly,on Tenebrio molitor pretreatment,and to determine moisture content standard below 4%.Secondly,using moderate of Soxhlet extractor method to extract oil in different temperature of different ways and analysising extraction rate of variance,and by using kjeldahl and BBC G-250 determinations of protein content before and after the degreasing.Results showed that considering oil and protein,the best combination of extraction rate was that the first time of the best temperature was 65 ℃ and the second of the optimum temperature was 45 ℃.The 65 ℃,45 ℃ was that oil extraction rate reached 19.99%,extraction rate of 16.12%,protein extraction yield of 3.68%,the concentration of 0.3032 mg/m L.