帕金森病患者外周血单核细胞CD14和TLR4中mRNA的表达水平

Expressions of CD14 mRNA and TLR4 mRNA in peripheral blood mononuclear cells in patients with Parkinson disease

目的研究帕金森病患者外周血单核细胞(peripheral blood mononuclear cell,PBMC)脂多糖受体(cluster of differentiation 14,CD14)和toll样受体4(toll-like receptor 4,TLR4)中m RNA的表达并探讨其在疾病发生发展中的临床意义。方法本实验共入组44例帕金森病患者和37例性别、年龄相匹配的健康对照者,并详细记录入组的44例帕金森病患者发病年龄、病程、性别,在关期时对帕金森病患者进行H-Y分期、UPDRSⅡ、UPDRSⅢ、NMSS评分,利用RT-PCR技术检测各研究对象PBMC中CD14和TLR4中m RNA的表达。结果帕金森病患者外周血CD14 m RNA表达水平(1.459±0.658)2^(-△△CT)、TLR4 m RNA(1.408±0.698)2^(-△△CT)表达较健康对照者((1.162±0.631)2^(-△△CT)、(1.122±0.557)2^(-△△CT))均显著增加(P均<0.05)。相关性分析显示帕金森病组CD14 m RNA的表达水平与反应帕金森病疾病严重程度的H-Y分期呈显著正相关(P<0.05)。结论帕金森病患者外周血CD14 m RNA表达与其H-Y分期正相关,提示CD14/TLR4的单核细胞所介导的固有免疫反应可能是帕金森病发生、发展的一个重要因素。

Objective To investigate the expression levels of the CD14 mRNA and TLR4 mRNA in peripheral blood mononuclear ceils from patients with Parkinson disease and to explore the clinical signicance. Methods For- ty-four patients with Parkinson disease and 37 healthy controls were recruited. We recorded age of onset, duration of ill- ness and sex of all recruited patients. PD patients were evaulated using the Hoehn-Yahr stages, Unified Parkinson Dis- ease Rating Scale （UPDRS） II and UPDRS II,non-motor symptoms scale （NMSS） on ＂off＂ time. Reverse transcrip- tion-polymerase chain reaction was performed to determine the expression levels of CD14 mRNA and toll-like receptor 4 （TLR4） mRNA. Results The expression levels of CD 14 mRNA（1.459±0.658） 2 and TLR4 mRNA （1.408±0.698） 2- was significantly up-regulated （P〈0.05） in Parkinson disease compared with controls（（1.162±0.631）2- （1.122±0.557） 2-）. In addition, there was positive correlation between the expression levels of CD14 mRNA in Parkinson dis- ease patients with the Hoehn-Yahr stages. Meanwhile, there was no significant correlation between the expression levels of CD14 mRNA and TLR4 mRNA with other clinical scores. Conclusions There is positive correlation between the expression levels of CD14 mRNA in Parkinson disease patients with the Hoehn-Yahr stages, indicating that CD14/TLR4 positive monocyte may be involved in the pathogenesis of the Parkinson disease.