微波-超声波促进固载辣根过氧化物酶降解苯酚特性

Properties of Increasing Phenol Degradation by Immobilized HRP Using Microwave and Ultrasonic Irradiation

以水热法合成的层状氢氧化氨基苯甲酸锌为载体,对辣根过氧化物酶(Horseradish peroxidase,HRP)进行了固定化。在微波-超声波联合处理工艺条件下研究了固载酶对苯酚废水的催化降解特性。通过紫外分析、Lineweaver-Burk双倒数作图、液相色谱等方法测定了固载酶催化活性、动力学常数,比较了不同条件下固定化HRP的苯酚降解率和储存稳定性。结果表明,在60 mmol/L苯酚溶液中,微波-超声波辐照条件下,固载酶对其降解率为74.4%,提高43.6%。微波-超声波辐照通过加速分解抑制剂的方式提高固载酶的活性,增加降解率,这种促进作用随着苯酚浓度增加而加强。连续使用7次后,辐照组的降解率依然为对照组的5.06倍。

Horseradish peroxidase （HRP） was immobilized on layered hydrogen peroxide, which was synthesized by hydrothermal method. Catalytic degradation characteristics of phenol wastewater by immobilized enzyme .was studied under the conditions of microwave and ultrasonic combined treatment. Immobilized enzyme catalytic activity was investigated by means of UV analysis and kinetic constants of HRP immobilization were determined by Lineweaver-Burk double-reciprocal plot method. A comparison to phenol degradation efficiency and storage stability of immobilized enzyme under different conditions was conducted with liquid chromatography method. The results showed that the degradation efficiency of the immobilized enzyme was 74.4% under microwave-ultrasonic irradiation conditions, which was increased by 43.6% when phenol concentration was 60 mmol/L. Microwave-ultrasonic irradiation enhances enzyme activity, and hence the catalytic efficiency, by accelerating the decomposition of the said inhibitors. It was also found that the acceleration effect strengthens with an increased phenol concentration. After continuous use seven times, the degradation efficiency of irradiation group is still 5.06 times of that of the control group.