HPLC法测定坐珠达西中西红花苷的含量

Content Determination of croci stigma in zuozhudaxi by HPLC

目的:建立测定坐珠达西中西红花含量的方法。方法:采用高效液相色谱法。色谱柱为Waters C18(250mm×4.6mm,5μm),流动相为甲醇-乙腈-0.05mol/L醋酸铵(40∶5∶55),检测波长为440nm;流速为1ml/min;柱温为30℃。结果:西红花苷Ⅰ的质量浓度在6.208μg/ml^62.08μg/ml范围内与其峰面积积分值呈良好线性关系(r=0.9997);精密度、稳定性、重复性的RSD<2%;平均加样回收率为98.31%,RSD=0.86%(n=6);西红花苷Ⅱ的质量浓度在3.072~30.72μg/ml范围内与其峰面积积分值呈良好线性关系(r=0.9999);精密度、稳定性、重复性的RSD<2%;平均加样回收率为97.69%,RSD=0.99%(n=6)。结论:该方法操作简单,结果准确,可用于坐珠达西中西红花苷的含量测定。

Objective To develop a method for the content determination of croci stigma in zuozhudaxi. Methods HPLC method was adopted. The determination was performed on Waters C18column（ 250 mm × 4. 6mm,5μm） with mobile phase consisted of methanol- acetonitrile- 0. 05 mol / L ammonium acetate（ 40∶ 5∶ 55） at the flow rate of 1ml / min. The detection wavelength was set at 440 nm,and column temperature was 30 ℃. Results The linear range of crocin Ⅰ were 6. 208 ～ 62. 08μg / ml（ r = 0. 9997） with an average recovery of 98. 31%（ RSD = 0. 86%,n = 6）; RSDs of precision,stability and reproducibility tests weve all lower than 2%. The linear range of crocin Ⅱ were 3. 072 ～ 30. 72μg / ml（ r = 0. 9999） with an average recovery of 97. 69%（ RSD = 0. 99%,n = 6）; RSDs of precision,stability and reproducibility tests weve all lower than 2%. Conclusion The method is simple,accurate,and can be used for the content determination of croci stigma in zuozhudaxi.