摘要
目的：建立高效液相色谱法（HPLC）测定消化胶囊中橙皮苷的含量。方法：采用十八烷基键合硅胶色谱柱（4.6mm×250mm,5μm）;以乙腈-水（20∶80）为流动相,流速为1.0ml/min;柱温：30℃;检测波长：284nm。结果：橙皮苷含量在10.15～81.20μg（r=0.9995）范围内与峰面积呈良好线性关系;平均回收率为98.50%（RSD为0.46%）。结论：本方法准确、快速、简便可靠,结果稳定,可用于消化胶囊中橙皮苷的含量测定。
Objective To establish high performance liquid chromatography（ HPLC） determination of hesperidin in digestive capsule. Methods Using octadecyl bonded silica gel chromatography column（ 4. 6mm × 250 mm,5μm）; With acetonitrile- water（ 0）were as mobile phase,flow rate of 1. 0ml / min; Column temperature： 30℃; detection wavelength 284 nm. Results Concentration of hesperidin in 10. 15 ～ 81. 20μg（ r = 0. 9995） range and peak area showed a good linear relationship. The average recovery rate was98. 5%（ RSD was 0. 51%）. Conclusion This method is simple and reliable,rapid,accurate and stable results and can be used for determination the content of hesperidin in digestive capsule.
出处
《中国民族民间医药》
2016年第7期19-20,共2页
Chinese Journal of Ethnomedicine and Ethnopharmacy
