“从脾论治”肝纤维化对模型大鼠相关因子水平影响实验研究

Treated from the Spleen of Liver Fibrosis Related Factor Influences on Model Rats of Experimental Research

目的:观察肝脾同治药物软肝颗粒对肝纤维化大鼠的抑制作用,并探寻其作用机制。方法:实验中将60只实验大鼠随机分为空白对照组、模型对照组、软肝颗粒组与活血化瘀4组。分别通过四氯化碳诱导成为肝纤维化模型大鼠,软肝颗粒组及活血化瘀组分别予软肝颗粒和大黄蛰虫丸灌胃,实验结束后检测各组大鼠行为学指标、肝脏病理组织学改变,以及HA、LN、PCⅢ、Ⅳ-C水平等指标,观察软肝冲剂对肝纤维化的治疗及逆转病程的作用。结果:软肝颗粒组及活血化瘀组HA、LN、PCⅢ及Ⅳ-C水平均低于模型对照组(P<0.01);软肝颗粒组HA、LN、PCⅢ及Ⅳ-C水平低于活血化瘀组(P<0.05)。结论:软肝颗粒对肝纤维化的抑制及逆转作用较活血化瘀法更具一定优势。

Objective：To observe the liver and spleen stagnation soft liver drug particles inhibition of liver fibrosis in rats and explore its action mechanism. Methods：80 experiment,the experimental rats were randomly divided into blank control group,model control group,soft liver granule group and promoting blood circulation to remove blood stasis four groups. Respectively in rats induced by carbon tetrachloride into liver fibrosis models,soft particles liver and promoting blood circulation to remove blood stasis group respectively with soft liver granule and rhubarb sting worm pill to fill the stomach,after the experiment test each behavior index of rats,pathological and histological changes of liver and HA,LN,PC Ⅲ and Ⅳ- C level indicators,such as soft liver granule on the treatment of liver fibrosis,and the function of reverse course. Results：The soft particle liver and promoting blood circulation to remove blood stasis group HA,LN,PC Ⅲ and Ⅳ- C level is lower than the model control group（P〈0.01）;Soft liver granule group of HA,LN,PC Ⅲ and Ⅳ-Clevel below the promoting blood circulation to remove blood stasis group（P〈0.05）. Conclusion：the soft liver granule on liver fibrosis inhibition and a reverse effect of promoting blood circulation to remove blood stasis method has more advantages.