一株犬源芽孢杆菌的分离鉴定

Isolation and Identification of A Strain of Bacillus from Dogs

为研究和开发新型宠物微生态制剂，解决耐药性、药物残留等问题，从健康犬新鲜粪便中分离获得1株具有较高抗逆性的菌株，命名为HEW—Q214，通过形态观察、生理生化特性和16SrDNA序列同源性分析，鉴定该菌株为枯草芽孢杆菌，通过抑菌试验和耐药性试验表明，HEW—Q214对大肠杆菌、金黄色葡萄球菌及沙门氏菌均有明显的抑菌效果，对大多数抗生素较敏感。

In order to research and develop new pet probiotics, and resolve drug resistance, drug residues and other issues, a strain with higher resistance isolated from fresh feces of healthy dogs ,named HEW- Q214 ,the strain was identified as Bacillus subtilis by morphological, physio- logical,biochemical characteristics and 16S rDNA sequence analysis. The inhibition test and drug test showed that HEW- Q214 had significant inhibitory effect to Escherichia coli, Staphylococcus aureus and Salmonella, and was more sensitive to most antibiotics.