基于荧光碳点的免疫荧光探针构建及其对大肠杆菌的特异性识别

Immunofluorescence probe based on fluorescent carbon dots and its application to the special recognition of Escherichia coli

目的将碳点(CDs)应用在免疫荧光探针成为取代传统荧光染料的新型标记物。方法通过微波加热方法制备高强荧光碳点,并通过EDC偶联法与大肠杆菌抗体结合形成复合免疫荧光探针,以大肠杆菌O157∶H7为检测模型进行特异性识别实验。结果碳点成功应用在免疫标记大肠杆菌O157∶H7,并可见多色荧光。结论免疫荧光探针成功地识别大肠杆菌O157∶H7,表明碳点可作为免疫荧光探针的荧光标记物,有望制成具有自主知识产权的新型低毒生物传感器。

Objective Carbon dots（ CDs） are an emerging carbon nano-material which is environmentally-friendly,economical,efficient and stable. Their fluorescence properties can match the semiconductor quantum dot. Moreover,CDs have more excellent biocompatibilities. The purpose of this experiment is to apply CDs to the fluorescent immune probe to make them a new label,which can replace the traditional fluorescent dyes. Methods Using microwave heating method,the high strength fluorescent carbon dots were prepared. Wtih the EDC coupling method,the high strength fluorescent carbon dots could bond with Escherichia coli antibodies to form a complex immune fluorescent probe. Specific recognition experiments were carried out in the model of E. coli O157∶ H7. Results CDs were successfully applied to immune recognition of E. coli O157∶ H7 and multicolor fluorescence was observed. Conclusion CDs can serve as a label of the fluorescent immune probe,and are expected to become a new type of low toxicity biosensor with independent intellectual property rights.