宫颈癌中MTSS1基因的表达及其临床意义

Expression of MTSS1 in cervical cancer tissue and its clinical significance

目的检测宫颈正常组织以及不同临床分期宫颈癌组织中转移抑制基因1(metastasis suppressor 1,MTSS1)的表达,分析该基因在各组织中的变化与临床病理因素的关系,并初步探讨该基因在宫颈癌发生发展及转移过程中的作用及分子机制。方法取2011年12月至2014年12月期间采集的103例宫颈组织,病理切片诊断患者宫颈组织类型,判断分化程度,应用实时荧光定量PCR(q-PCR)和Western印迹法检测不同宫颈组织中MTSS1基因及其蛋白的表达水平。结果 q-PCR结果提示,ⅡB-Ⅳ期宫颈癌组MTSS1基因表达量明显高于正常宫颈组及Ⅰ-ⅡA期宫颈癌组,3组间均有明显差异(P=0.000);Western印迹检测MTSS1蛋白在正常宫颈组织中的阳性表达率为23.3%,在宫颈癌组织中为53.3%,两组间差异明显(P=0.002);MTSS1在宫颈癌组织中的表达与年龄、分化以及有无淋巴结转移均无明显相关性(P>0.05),而在临床分期中,ⅡB-Ⅳ期MTSS1蛋白表达率明显高于Ⅰ-ⅡA期的宫颈癌组织(P=0.005)。结论 MTSS1的表达与宫颈癌的临床分期呈正相关趋势,该基因可能在宫颈癌的发生发展中起着重要的作用。

Objective To detect the expression of metastasis sappressor 1（ MTSS1） gene in cervical cancer tissue and to clarify its association with cervical cancer. Methods Totally 103 cases of cervical tissue were collected between Dec2011 and Dec 2014 and classified according to biopsy and stage. Q-PCR and Western blotting were used to detect the expression of MTSS1 in normal cervical tissue and in different clinical stages of cervical cancer tissue. Results The expression of MTSS1 in ⅡB-Ⅳ stages of cervical cancer tissue was significantly higher than that of normal tissue or Ⅰ-ⅡA stages through q-PCR（ P = 0. 000）. Western blotting results showed that MTSS1 was positively expressed in normal cervical tissue at a rate of 23. 3% or 53. 3% in cervical cancer tissue. Moreover,the expression of MTSS1 was poorly correlated with age,tumor differentiation and lymphnode metastasis in cervical cancer tissue（ P〉0. 05）. The protein level of MTSS1 expressed in ⅡB-Ⅳ stages was significantly higher than that ofⅠ-ⅡA stages（ P = 0. 005）. Conclusion The expression of MTSS1 indicates the clinical stage of cervical cancer,suggesting that MTSS1 may play an important role in the development of cervical cancer.