摘要
本文介绍一种简单、快速的提取和纯化大肠杆菌耐热肠毒素(ST)的方法,纯化过程包括:硫酸铵沉淀,Sephadex G-25 Bio-Gel P-4凝胶过滤。纯化后的纯毒素比活性为5×10~5鼠单位/mg蛋白。肠毒素被纯化167倍,回收率为33.7％。
A simple rapid procedure for extracton and purification of the heat-stable enterotoxin of E. coli (ST) has been described. The purification scheme involved ammonium sulfate precitation,Sephadex G-25 and Bio-Gel P-4 gel filtrations. The specific activity of purified ST preparation was 5×10 mouse units/mg protein. This enterotoxin was purified to approximately 167-fold and the recovery was 33. 7%.
出处
《中国人兽共患病杂志》
CSCD
北大核心
1991年第3期5-7,共3页
Chinese Journal of Zoonoses
关键词
大肠杆菌
耐热肠毒素
纯化
特性
Enterotoxigenic E. coli, Heat-stable enterotoxin, Purification.
