PRDM14基因在猪早期胚胎和主要器官中的表达

The expressions of PRDM14 gene in porcine early embryos and main organs

PRDM14是一种具有调控基因表达功能的转录因子,在早期胚胎发育过程中可以促进多能性基因或抑制分化基因的表达,从而影响到整个生命体的发育进程。为了研究转录因子PRDM家族中PRDM14基因在猪早期胚胎过程中的表达变化以及在新生仔猪主要器官中的表达差异,本试验以猪为研究对象,首先利用预测序列设计引物,通过序列比确定基因,然后利用荧光定量PCR等技术,对PRDM14基因在猪早期胚胎各时期及新生仔猪主要器官中的表达进行了研究。结果表明:PRDM14基因在卵母细胞MⅡ期表达较高,经过孤雌活化后,在2细胞阶段有所下降,之后随发育表达逐渐升高,而在囊胚阶段又有所降低。在新生仔猪主要器官,睾丸和卵巢中表达较高,在其他器官表达较低。结果表明,PRDM14基因在猪早期胚胎及生殖器官发育方面发挥重要作用。

PRDM14 is a transcription factor, which regulates gene expression. It can promote the ex- pression of pluripotency genes or inhibit differentiation in early embryo development, thus affect the whole life of development process. PRDM14 gene is a member of transcription factor PRDM family. This study was conducted to investgate PRDM]4 gene expression changes in porcine early embryos as well as the expression differences in main organs in newborn piglets. Firstly, designed three pairs of primers to get the products of this gene, then used RT-PCR to detect the expression of PRDM14 in early devolpoment embryos and the main orgens of new born piglets. The results showed that the PRDM14 gene expressed highly at M II oocytes and decreased in the 2-cells embry- os derived from parthenogenetic activation. Following the development,the expression of PRDM14 was increased gradually, but decreased in blastocysts. In the main organs of newborn piglets, PRDM14 gene expressed highly testis and ovaries,lowly in other organs. The results indicated that PRDM14 gene plays an important role in porcine early embryo and reproductive organ development.