山东地区健康人群腺病毒稀有血清型感染状况调查分析

Investigation and analysis on infection situation of rare adenovirus serotype among normal population in Shandong area

目的调查山东地区健康人群中腺病毒不同血清型的感染状况,筛选出流行水平较低的腺病毒血清型,用于腺病毒载体构建。方法利用腺病毒不同血清型标准株,以ELISA方法检测健康人群中不同腺病毒血清型的中和抗体。结果山东地区18～70岁的健康人群中腺病毒总抗体的阳性率为95.2%。其中Adv3、Adv5、Adv7、Adv11、Adv26、Adv35、Adv48、Adv49中和抗体的阳性率分别为：69.8%、71.0%、61.0%、9.4%、2.2%、5.8%、0.8%、1.6%。Adv11、26、35、48、49 5个血清型的阳性率远低于Adv3、5、7型（P〈0.01）。结论山东地区腺病毒的总感染率较高,不同血清型之间有显著差异,其中Adv26、48、49型腺病毒感染率较低,可用于腺病毒载体的构建。

Objective To investigate the infection status of different adenovirus serotypes of adenovirus among normal population in Shandong area in order to screen out the serotypes of low prevalence level for the use in the adenoviral vector construction.Methods Serum levels of neutralizing antibodies against different serotypes of adenovirus were determined with ELISA by using different adenovirus serotype standard strains.Results The positive rate of total adenovirus antibodies in the normal population aged 18-70 years old in Shandong area was 95.2%,in which the antibody positive rates of Adv3,Adv5,Adv7,Adv11,Adv26,Adv35,Adv48 and Adv49were 69.8%,71.0%,61.0%,9.4%,2.2%,5.8%,0.8% and 1.6%,respectively.The positive rates of Adv11,26,35,48,49 neutralizing antibodies were significantly lower than those of Adv3,5,7serotypes（P〈0.01）.Conclusion The adenovirus total infection rate is high in Shandong area.The infection rates of different serotypes are different,in which Adv26,48,49 serotypes have lower infection rates and can be used for constructing the adenovirus vector.