原发性高血压患者不同中医证候与血管紧张素原基因7个位点多态性的相关性研究

Study on Correlation between Angiotensinogen Gene Polymorphism and Different Syndromes of Essential Hypertension in Chinese Medicine

目的:通过不同证型原发性高血压(essential hypertension,EH)患者血管紧张素原(angiotensinogen,AGT)基因7个位点多态性研究,探寻影响EH证候的功能基因位点,从分子遗传学的角度初步揭示EH的证候实质。方法:选择4型EH患者400例,正常人100例。采用多重高温连接酶检测反应基因分型技术(improved multiple Ligase Detection Reaction,imLDR^(TM))检测AGT基因多态性。结果:AGT rs5049基因型在各中医证候和对照组比较有显著性差异(P<0.05),其中CC基因型在痰湿壅盛组和阴阳两虚组最高。结论:EH中医证候与AGT基因多态性存在关联性,AGT rs5049在安徽合肥汉族人EH发病中具有重要意义,CC基因型可能使痰湿壅盛证和阴阳两虚证的发生风险增加。

Objective： To explore the functional gene locus which affect the syndromes of essential hypertension（EH） and reveal the essence of the syndromes of EH from the perspective of molecular genetics by studying the distribution of the polymorphism of seven gene locus of EH patients with different syndromes in traditional Chinese medicine. Methods ：400 EH patients from four types of syndromes in Chinese medicine and 100 healthy control group were selected. The polymorphism of AGT gene was detected by applying improved multiple Ligase Detection Reaction （imLDRTM）. Results： AGT rs5049 genotype in EH paitents group was differennt from control,CC genotype was the highest in excessive phlegmatic dampness type and deficiency of both yin and yang. Conclusion： The syndrome of EH in Chinese medicine is associated with the AGT gene polymorphism. AGT rs5049 is closely associated with the pathogenesis of EH of Han nationality in the region of Hefei in Anhui Province. CC genotype may raise the probability of the formation of excessive phlegmatic dampness type and asthenia of both yin and yang.