中国沿海多鳞四指马鲅(Eleutheronema rhadinum)与四指马鲅(E.tridactylum)形态与遗传位点差异分析

MORPHOLOGICAL VARIATIONS AND GENETIC LOCI VARIATIONS BETWEEN ELEUTHERONEMA RHADINUM AND E. TRIDACTYLUM ALONG CHINA COAST

多鳞四指马鲅(Eleutheronema rhadinum)与四指马鲅(Eleutheronema tridactylum)是四指马鲅属(Eleutheronema)中的名贵经济鱼类,具有良好的养殖开发前景。本文对中国沿海多鳞四指马鲅与四指马鲅群体的26个形态特征值进行了多变量比较分析,提取了7个主成分,累计贡献率达到91.112%,前三个主成分贡献率分别为28.759%、21.467%、15.469%。多鳞四指马鲅与四指马鲅形态差异主要体现在头背部、尾柄部、背腹轴及头尾轴的特征。利用4个形态学参数建立了2群体判别公式,综合判别准确率高达100%,可作为多鳞四指马鲅与四指马鲅物种资源的初步判定方法。另外,利用简化基因组测序开发出多鳞四指马鲅与四指马鲅SNP标记69207个、In Del标记12884个,丰富了四指马鲅属鱼类遗传结构研究数据。本研究为进一步开展四指马鲅数属物种资源评估与保护、选育、功能基因研究提供基础资料。

Eleutheronema rhadinum and E. tridactylum are economically valuable species. We analyzed 26 morphological traits in multivariate comparison method for the two species distributed along China coast. Seven principle components were extracted and their accumulative contribution rate was 91.112%, of which the first three principle components contributed 28.759%, 21.467%, and 15.469%. Morphological variations between E. rhadinum and E. tridactylum were substantiated on back of head, caudal feature, dorso-ventral axis, and anterior-posterior axis. Discriminant functions of two populations based on 4 morphological parameters were proposed, with which overall discrimination accuracy reached 100%. These functions were used to analyze species resource of the fish. Meanwhile, using simplified genome sequencing technology, SNP and InDel between E. rhadinum and E. tetradactylum populations were obtained for 69207 and 12884, respectively, which enriched information of the genetic structure field. This study may provide a support in resource estimation and protection, selective breeding, and functional gene research of Eleutheronema genus.