七氟醚对糖尿病小鼠认知功能的影响

Effects of sevoflurane on cognitive function in diabetic mice

目的观察吸入七氟醚对糖尿病小鼠认知功能的影响。方法单剂量腹腔注射链脲佐霉素（150mg／kg）诱导小鼠糖尿病模型，将造模成功（空腹血糖〉11．1mmol／L）的20只小鼠随机分为糖尿病组（DM组）和七氟醚糖尿病组（DM＋S组）；另设对照组（C组）及七氟醚组（S组），每组10只。C组吸入空气，DM组吸入空气，S组吸入1．5％七氟烷6h，DM＋S组吸入1．5％七氟醚6h。24h后，分别用跳台法、避暗法记录遗忘小鼠错误次数（errortimes，ETs）、跳台潜伏期（stepdown lataney，SOL）和步入潜伏期（stepthroughlatancy，STL）。结果DM＋S组SDL和STL分别为（98±9）、（32±32）s，与C组、DM组、s组比较均下降（P〈0．05）；跳台实验和避暗实验的ETs分别为（4．7±O．5）次和（3．1±2．1）次，与C组、DM组、s组比较均增加（P〈0．05）。结论吸入七氟醚对糖尿病小鼠短期内的认知功能有影响，加重糖尿病对认知功能的损害。

Objective To investigate the effects of sevoflurane on cognitive performance in diabetic mice. Methods Diabetic mice induced by a single Intraperitoneal injection of streptozotocin （150 mg/kg body weight） were used as animal model of diabetes. All animals were randomly divided into four groups, among this, diabetic mice with hyperglycemia （〉11.1 nmol/L） divided into two groups, including diabetic mellitus group（group DM） and diabetic mellitus and sevofurane group（group DM＋S）. Other groups are control group （group C） and sevoflurane group （group S）（10 mice/group）. Coup C and group DM inhaled air for 6 h, group S and group DM＋S inhaled 1.5% sevoflurane. After 24 h, the error times （ETs）, step down latancy （SDL） and step through latancy （STL） were observed and recoded in the step-down test and step-through test. Results The SDL and STL of group DM＋S （98±9） s and （32±32） s are decreased compared with group C, group DM and group S. The Ets of the step-down test and step-through test （4.7±0.5） and （3.1± 2.1） are increased compared with group C, group DM and group S. Conclusions Sevoflurane can exert influence on the shortern memory in diabetic mice.