摘要
目的建立微生物法测定肠内营养粉剂中微量维生素B_(12)的含量。方法以莱士曼氏乳酸杆菌为实验菌,在测定用培养基中供给除维生素B_(12)以外的所有营养成分,(36±1)℃培养19~20 h,采用比浊法测定。结果维生素B_(12)在0.001~0.010 ng/mL浓度范围内线性关系良好,肠内营养粉剂中维生素B_(12)的含量为2.9μg/100 g。结论该方法检测结果准确、可靠,适用于肠内营养粉剂中微量维生素B_(12)的含量测定。
Objective To establish a method for the determination of trace vitamin B_(12) in enteral nutrition powder by microbiological method.Methods The test organism was Lactobacillus leichmamii.The medium without vitamin B_(12) was used as assay medium.The sample were incubated for 19~20 h at the temperature of(36±1) ℃ and measured by microbial turbidimetric method.Results Vitamin B_(12) had a good linear relationship in the range of 0.001~0.010 ng/mL,and the content of vitamin B_(12) in enteral nutrition powder was2.9 μg/100 g.Conclusion This method is accurate and reliable,and suitable for the assay of trace vitamin B_(12)in enteral nutrition powder.
出处
《食品安全质量检测学报》
CAS
2016年第3期893-897,共5页
Journal of Food Safety and Quality