高效液相色谱法测定肉苁蓉中京尼平苷酸的含量

Determination of geniposidic acid in herba cistanche by high performance liquid chromatography

目的建立一种高效液相色谱法测定肉苁蓉中京尼平苷酸含量的分析方法。方法采用60%甲醇为溶剂,超声提取样品。选择Agilent ZORBAX SB-C_（18）色谱柱（4.6mm×250 mm,5μm）,流动相为甲醇和0.5%乙酸水溶液梯度洗脱,流速1.0 mL/min,柱温30℃,检测波长237 nm。结果京尼平苷酸在5.0~100.0μg/mL范围内线性关系良好（r=0.9999）,平均回收率在95%~101%之间,RSD 0.55%~1.96%。肉苁蓉、管花肉苁蓉和盐生肉苁蓉中京尼平苷酸的含量存在差异。结论本方法快捷、简便、准确,可用于测定肉苁蓉中京尼平苷酸的含量。

Objective To establish a method for the determination of the content of geniposidic acid in herba cistanche by high performance liquid chromatography（HPLC）.Methods Samples were extracted ultrasonically with 60%methanol as the solvent.An Agilent ZORBAX SB-C_（18） column（4.6 mm×250 mm,5 μm）was used with the mobile phase consisted of methanol and 0.5%acetic acid.The flow rate was 1.0 mL/min,and the column temperature was set at 30 ℃.The UV detection wavelength was 237 nm.Results There was a good linearity when the concentration of geniposidic acid was 5.0~100.0 mg/mL（r=0.9999）.The average recovery was 95%~101%,and the RSD was 0.55%~1.96%.There were differences between the 3 different sources of hebra cistanehe（Cistanche deserticola,Cistanche tubulosa and Cistanche salsa） in geniposidic acid content.Conclusion This method is simple and accurate,and it can be used for determination of geniposidic acid in herba cistanche.