体外心肌细胞缺氧/复氧模型损伤及参元丹干预的影响

The Effect of Shen Yuan Dan on the Vitro Myocardial Model Cells with Hypoxia/reoxygenation Injury

目的:建立体外培养乳鼠心肌细胞缺氧/复氧模型,观察益气逐瘀组方参元丹(SYD)对模型心肌细胞存活率和细胞损伤程度的影响,以进一步探讨其改善冠心病围手术期心肌损伤的作用机制。方法:体外培养大鼠乳鼠原代心肌细胞,建立缺氧复氧细胞模型并予分组处理,CCK法检测心肌细胞存活率,酶法检测各组细胞培养上清液中磷酸肌酸激酶(CPK)、乳酸脱氢酶(LDH)的含量。结果:模型组存活率低于正常组(P<0.05),而CPK、LDH释放量高于正常组(P<0.05);SYD含药血清组、PDTC+SYD组以及PDTC+空白血清组细胞存活率均高于模型组和空白血清组(P<0.05),细胞酶释放量均低于模型组和空白血清组(P<0.05),此3组间的数据比较,差异不具有统计学意义。结论:参元丹能够提高心肌细胞缺氧/复氧损伤模型的细胞存活率,降低细胞损伤程度;其机制可能通过抑制核因子NF-κB及其介导的氧化应激和炎性反应降低细胞损伤;提示参元丹可能具有围手术期心肌损伤的保护作用。

Objective： Culturing the neonatal rat myocardial cells＇ hypoxia / reoxygenation modle in vitro and observe the effects of Yiqi Zhuyu Formula（ Shenyuandan,SYD） on the survival rate and the degree of myocardium cells injury biomarkers,and thus to further discuss the its relieving mechanism on PMI. Methods： Neonatal rat myocardial cells were cultured in vitro to establish the hypoxia / reoxygenation model. Then use CCK-8 assay to detect the survival rate and enzymatic assay to detect the levels of CPK and LDH on each cell culture supernatant. Results： Compared with the normal group,the survival rate of the model group is lower（ P〈0. 05） and the CPK and LDH release quantity is higher（ P〈0. 05）. The survival rate of SYD drug-containing serum group,PDTC ＋ SYD and PDTC ＋ blank serum group were higher than those of model group and blank group（ P〈0. 05）; serum cell enzyme（ CPK,LDH） release quantity are lower than those of the model group and blank serum group（ P〈0. 05）,while there was no statistical difference among the three groups. Conclusion： Shen Yuan Dan can improve the cell survival rate of myocardial cell hypoxia / reoxygenation injury model and relieve cell injury. This may because it can reduce cell damage through inhibiting NF-κB and its mediated oxidative stress and inflammatory response. It indicates that Shen Yuan Dan could protect myocardium cells.