水飞蓟宾微粒分散体对CCl_4所致小鼠急性肝损伤的预防作用研究

The Effect of Silybin Micro-particle Dispersion on CCI_4 Induced Acute Liver Injury Mice

目的:研究水飞蓟宾微粒分散体对CCl4所致小鼠急性肝损伤的预防作用,为水飞蓟宾微粒分散体的临床药效研究提供实验依据。方法:采用CCl4诱发雄性ICR小鼠急性肝损伤为实验动物模型,比较了正常组、模型组、低剂量组、中剂量组、高剂量组、超高剂量组、原药粉组各组血及肝中AST、ALT、SOD、HYP、GSH-PX含量的差异。结果:中剂量组和原药粉组2组小鼠的AST、ALT、SOD、HYP、GSH-PX含量相比较,差异无统计学意义;超高剂量组的ALT、AST、HYP含量与空白组含量差异无统计学意义。结论:1/10原药粉口服给药剂量的水飞蓟宾微粒分散体静脉给药后预防肝纤维化的作用与水飞蓟宾原药粉口服给药剂量的作用相当,说明将水飞蓟宾制成水飞蓟宾微粒分散体后静脉给药可以减少单次或总的用药剂量,降低机体的耐药程度;尾静脉给药40 mg/kg,预防7 d后造模,小鼠肝组织抵抗能力增加,基本不受CCl4的影响。

Objective： To observe the effect of silybin particle dispersion on CCI4 induced acute liver injury and to provide support for its clinical usage. Methods： To make acute liver injury models by CCI4 and then compared the differences of ALT AST,SOD,HYP,GSH-PX in blood and liver in the normal group,model group,low dose group,middle dose group,high dose group,super high dose group,and the crude powder group. Results： ALT AST,SOD,HYP,GSH-PX in the middle and crude powder group were of no significant difference. ALT,AST,HYP in the super-high and blank control group were of no significant difference.Conclusion： The preventive effect of liver fibrosis were equivalent between the 1 /10 original powder dosage of silybin particle dispersion by intravenous administration and in the crude powder dosage,which illustrated that silybin particle dispersion by intravenous administration can reduce the single and the total administration dosage and the degree of resistance to the body. Besides,it indicated that the intravenous administration,40 mg·kg-1,prevention of 7 days model mice were largely unaffected by CCl4 and their liver resistance capability were improved.