纳米雄黄抑制人乳腺癌MCF-7细胞上皮间质转化的机制探讨

The Mechanism of Nanometer Realgar to Inhibit the Epithelial Mesenchymal Transition of Breast Cancer MCF-7 cells

目的:观察纳米雄黄对乳腺癌MCF-7细胞上皮间质转化的影响,并探讨其相关机制。方法:体外培养乳腺癌MCF-7细胞,不同浓度纳米雄黄溶液干预,并设立对照组,免疫组化实验检测各组细胞E-cad、HIF-1α表达情况,RT-PCR检测SnailmRNA表达情况。结果:各纳米雄黄组E-cad表达阳性率均明显高于对照组(P<0.01),而HIF-1α表达阳性率均低于对照组(P<0.01);对照组、纳米雄黄低、中、高剂量组、阿霉素组的SnailmRNA相对表达量依次为(52.16±3.87)、(30.10±2.48)、(47.08±3.45)、(41.42±4.35)、(36.07±2.45)。结论:纳米雄黄可通过上调E-cad表达、下调HIF-1α及Snail mRNA表达逆转乳腺癌MCF-7细胞上皮间质转化发挥抗肿瘤转移作用。

Objective： To explore the effect of nanometer realgar affecting epithelial mesenchymal transition of the breast cancer MCF-7 cells and its possible mechanism. Methods： Breast cancer MCF-7 cells were cultured in vitroand and use different doses of nanometer realgar to intervene. A control group was made. To test the expression of E-cad and HIF-1α,RT-PCR to detected the expression of Snail mRNA. Results： Compared with the control group,the E-cad positive rates of the nanometer realgar group were higher than the control group（ P〈0. 01）; the HIF-1α positive rates of the nanometer realgar group were less than the control group（ P〈0. 01）; the control group,nanometer realgar low,medium and high dose groups,doxorubicin group of Snail mRNA relative expression levels were（ 52. 16 ± 3. 87）,（ 30. 10 ± 2. 48）,（ 47. 08 ± 3. 45）,（ 41. 42 ± 4. 35）,（ 36. 07 ± 2. 45） respectively.Conclusion： Nanometer realgar can inhibit the migration and invasion of breast cancer MCF-7 cells,and it has anti-tumor metastasis. Nanometer realgar can resist tumor through reversing EMT by raising the level of E-cad and reducing the level of HIF-1α and Snail mRNA.