益气养阴、活血通络法对糖尿病兔血浆TXB_2、6-keto-PGF_(1α)、GMP-140含量和脑组织的影响

Effects of qi- tonifying and yin- nourishing therapy and/or blood- activating and collateral- dredging therapyon plasma TXB_2,6- keto- PGF_(1α),and GMP-140 levels and brain tissue in rabbits with diabetes mellitus

目的:观察中医益气养阴法、活血通络法及益气养阴+活血通络法3种治法对糖尿病(DM)大耳白家兔模型血浆TXB2、6-keto-PGF1α、GMP-140含量和脑组织的影响,比较3种治法防治DM脑血管病变的作用,并探讨其作用机制。方法:用静脉注射四氧嘧啶的方法制作糖尿病动物模型,随机分为益气养阴+活血通络组(即综合组)、益气养阴组、活血通络组及达美康组,并设正常对照组。药物干预30d后,检测比较各组大鼠体质量、血糖以及血浆中TXB_2、6-keto-PGF1α、GMP-140含量;对大脑中动脉处脑组织进行肉眼、常规病理及电镜检查。结果:与正常对照组比较,模型组兔体质量明显减轻,血糖升高,血浆中TXB_2、GMP-140含量明显升高,6-Keto-PGF1α含量明显减低,差异有统计学意义(P<0.05或P<0.01)。经用益气养阴+活血通络方、活血通络方、西药达美康治疗后,模型动物的TXB2与GMP-140含量明显降低,6-keto-PGF1α明显提高,差异有统计学意义(P<0.05或P<0.01),而单用益气养阴方则无明显作用。电镜下模型动物大多可见到血管内皮细胞的线粒体、内质网及胞浆溶解等改变,少数可见有脑实质神经突起变性,给药组动物出现例数均较模型对照组少,以综合组及达美康组出现最少。结论:益气养阴+活血通络方能较好地防治家兔糖尿病脑血管并发症的形成。

Objective： To observe the effects of three traditional Chinese medicine treatments,qi- tonifying and yin nourishing therapy,blood- activating and collateral- dredging therapy,and a combination of the two therapies,on plasma levels of thromboxane B2（ TXB2）,6- keto- prostaglandin F1α（ 6- keto- PGF1α）,and granular membrane protein- 140（ GMP- 140） and brain tissue in big- ear white rabbits with diabetes mellitus（ DM） and to compare the three treatments for DM with cerebrovascular diseases. Methods： A rabbit model of DM was established by intravenous injection of tetraoxypyrimidine. The model rabbits were randomly divided into model group,qi- tonifying and yin- nourishing therapy plus blood- activating and collateral- dredging therapy group（combination therapy group）,qi- tonifying and yin- nourishing therapy group,blood- activating and collateral- dredging therapy group,and Diamicron group; in addition,normal rabbits were selected as the normal control group. After 30 days of medication,these groups were compared in terms of body weight,blood glucose,and plasma levels of TXB2,6- keto- PGF1α,and GMP- 140; the brain tissue at the middle cerebral artery was observed with the naked eye,by pathological examination,and under an electron microscope. Results： Compared with the normal control group,the model group had a significantly reduced body weight,significantly increased blood glucose and plasma TXB2 and GMP- 140 levels,and a significantly reduced plasma 6- Keto- PGF1α level（ P〈0. 05）. Compared with the model group,the combination therapy group,blood- activating and collateral- dredging therapy group,and Diamicron group had significantly reduced plasma TXB2 and GMP- 140 levels,and a significantly increased plasma 6- keto- PGF1α level（ P〈0. 05 or P〈0. 01）. However,the qi- tonifying and yin- nourishing therapy group showed no significant improvements in the above indices compared with the model group. The electron microscopy showed lysis of mitochondria,endoplasmic reticulum,and cytoplasm in vascular endothelial cells in most model rabbits and nervous process degeneration in brain parenchyma in few rabbits; the treated groups had fewer animals with the above changes than the model group,and the combination group and Diamicron group had the fewest animals. Conclusion： A combination of qi- tonifying and yin-nourishing therapy and blood- activating and collateral- dredging therapy is effective in preventing and treating cerebrovascular complications in rabbits with DM.