尼可地尔预处理对大鼠缺血再灌注心肌mtDNA4834bp片段缺失的影响

Effect of Nicorandil Preconditioning on mt DNA 4834 bp Deletion of Myocardial Ischemia-reperfusion Injury in Rats

目的:探讨尼可地尔预处理对大鼠缺血再灌注心肌mt DNA4834 bp缺失突变的影响。方法:体重200 g-300 g,7-9周龄健康雄性Sprague-Dawley(SD)大鼠50只,随机平均分为5组,假手术(Sham)组、心肌缺血再灌注损伤(MIRI)组、缺血预处理(IPC)组、尼可地尔(Nic)组、尼可地尔+5羟基葵酸钠(Nic+5-HD)组。建立心肌缺血再灌注模型后取心肌组织,提取mt DNA,应用巢式PCR法定性检测mt DNA4834 bp片段缺失突变。结果:MIRI组、IPC组、Nic组和Nic+5-HD组突变频率分别为100%、20%、30%和89%。Sham组未检测到mt DNA4834 bp缺失突变。Nic组mt DNA4834 bp片段缺失明显低于MIRI组和Nic+5-HD组(P<0.05),与IPC组比较差异无统计学意义(P>0.05)。结论:尼可地尔预处理可降低大鼠MIRI后mt DNA4834 bp片段缺失突变,改善线粒体能量衰竭。

Objective： To investigate the effect of nicorandil preconditi oning on mitochondiral DNA 4834 bp deletion of myochondial ischemia-reperfusion injury in rats. Methods： Weighing 200 g-300 g, aged between 7-9 weeks fifty healthy male Sprague-Dawley（SD） rats were random Ly allocated into 5 groups（n=10 each）： group Sham, group MIRI,group IPC, group Nic and group Nic＋5-HD. The animals were sacrificed and myochondial tissues were collected, mitochondiral DNA was isolation. The mitochondrial DNA4834 bp deletion mutation was detected by nested-PCR（nested polymers chain reaction） technique. Results： The frequence of the mitochondrial DNA 4834 bp deletion mutation of MIRI group, IPC group, Nic group and Nic＋5-HD group were 100%, 20%, 30%, 89%.The mt DNA4834 bp mutation frequency of Nic group was significantly lower than MIRI group and Nic＋5-HD group（P〈0.05）, there were no significant difference between Nic group and IPC group（P〉0.05）. Conclusion： Nicorandil precondition decrease the mt DNA4834 bp deletion mutation of rats.