毛花苷C促进肝癌SMMC-7721细胞凋亡及抑制survivin的表达

Lanatoside C promotes apoptosis and inhibits survivin expression in hepatocarcinoma SMMC-7721 cells

目的:通过毛花苷C作用于人肝癌SMMC-7721细胞,研究其对SMMC-7721细胞增殖的影响初步探讨其作用机制.方法:使用不同浓度毛花苷C干预S M M C-7721细胞,通过细胞增殖实验及克隆形成实验,检测毛花苷C对SMMC-7721细胞增殖的作用;通过流式细胞仪检测毛花苷C对SMMC-7721细胞周期和凋亡的影响;采用Western blot技术分析细胞凋亡抑制基因survivin的表达.结果:毛花苷C对SMMC-7721细胞增殖有明显的抑制作用,各加药组与对照组相比差异有统计学意义(P<0.01),并呈现剂量-效应相关关系;流式细胞术显示,毛花苷C将S M M C-7721细胞阻滞于S期,并诱导其凋亡;Western blot检测结果显示毛花苷C下调SMMC-7721细胞内survivin蛋白的表达.结论:毛花苷C明显抑制SMMC-7721细胞增殖,将细胞阻滞在S期并诱导其凋亡.该机制可能与下调survivin的蛋白表达有关.

AIM： To investigate the effect of lanatoside C on the proliferation of human hepatocarcinoma SMMC-7721 cells and to explore the underlying mechanism.METHODS： SMMC-7721 cells were treated with lanatoside C. Then cell proliferation assay and colony formation assay were applied to detect the cell proliferation. The effect of lanatoside C on the cell cycle and apoptosis of SMMC-7721 cells were detected by flow cytometry. Western blot assay was used to detect survivin protein expression.RESULTS： Compared with control cells, lanatoside C significantly inhibited the proliferation of SMMC-7721 cells（P 0.01） in a dose-dependent manner. Results of flow cytometry indicated that lanatoside C arrested SMMC-7721 cells at the S phase and induced their apoptosis. Western blot assay showed that lanatoside C down-regulated the expression of survivin protein in SMMC-7721 cells.CONCLUSION： Lanatoside C could inhibit the proliferation of SMMC-7721 cells obviously, arrest the SMMC-7721 cells at S phase and induce their apoptosis. The mechanism may be associated with the down-regulation of survivin expression.