醋白芍、炙甘草及其配伍载体中药效组分变化规律分析

Analysis of Different Active Components in Radix Paeoniae Alba( Stir-baked with Vinegar),Glyycrrhizae Radix Et Rhzoma( Stir-baked with Honey) and their Drug Pairs

目的:研究醋白芍、炙甘草及其配伍载体中醋白芍及炙甘草药效组分的变化规律。方法:以醋白芍、炙甘草单味药及其药对为研究对象,采用水煎煮法制备供试品溶液,采用HPLC法测定。结果:醋白芍4种药效组分(氧化芍药苷、芍药内酯苷、芍药苷、苯甲酰芍药苷)含量分别为:醋白芍(1.07±0.12、0.36±0.02、2.00±0.21、0.37±0.03)mg/m L;醋白芍-甘草(1.89±0.11、0.86±0.05、3.25±0.13、0.52±0.03)mg/m L;醋白芍-炙甘草(1.90±0.09、0.87±0.04、3.30±0.24、0.52±0.01)mg/m L。炙甘草五种药效组分(甘草酸、甘草苷、甘草素、异甘草苷、异甘草素)含量分别为:炙甘草(6.53±0.25、2.17±0.33、0.52±0.21、0.48±0.23、0.09±0.03、9.79±0.65)mg/m L;炙甘草-焦白芍(5.13±0.32、2.11±0.21、0.47±0.12、0.57±0.22、0.12±0.02、8.40±0.75)mg/m L;炙甘草-炒白芍(6.47±0.33、2.67±0.23、0.56±0.23、0.60±0.23、0.12±0.01、10.42±0.78)mg/m L;炙甘草-醋白芍(8.76±0.35、3.10±0.43、0.79±0.32、0.76±0.34、0.15±0.05、13.56±0.99)mg/m L。结论:配伍影响或改变了药效组分的物理、化学性质,进而改变了药效组分的溶出,最终改变了药效组分在复方汤剂中存在状态。中药治疗作用的关键在于药效组分,包括药效组分的组成及各药效组分之间的比例。药效组分的组成不同,疗效也不同;药效组分的组成相同,但比例不同,疗效也不一样。不同组成的药效组分或不同比例的药效组分分别代表不同的中药,具有不同的临床疗效,其根源在于药效组分的不同,应加以区分。因此,对中药药效组分的研究与探讨是解析中药配伍机制的关键。

Objective： To explore different active chemical components in Radix paeoniae alba（ stir-baked with vinegar）,Glyycrrhizae radix（ stir-baked with honey） and their drug pairs. Methods： Water boiled Radix paeoniae alba,Glyycrrhizae radix respectively and use HCPL method to test their solution. Results： The four active constitutes（ Oxypaeoniflorin,Albiflorin,Paeoniflorin,Benzoylpaeoniflorin） were [1. 07 ＋ 0. 12,0. 36 ＋ 0. 02,2. 00 ＋ 0. 21,0. 37 ＋ 0. 03] in Radix paeoniae alba（ vinegar baked）; [1. 89 ＋ 0. 11,0. 86 ＋ 0. 05,3. 25 ＋ 0. 13,0. 52 ＋ 0. 03] in Radix Paeoniae alba（ vinegar baked）-Glyycrrhizae radix and [1. 90 ＋ 0. 09,0. 87 ＋ 0. 04,3. 30 ＋ 0. 24,0. 52 ＋ 0. 01] in Radix paeniae alba-Glyycrrhizae radix（ honey-fried）; The five active constituents（ Glycyrrhizic acid,Liquiritin,Liquiritigenin,Isoliquiritin,Isoliquiritigenin） were[6. 53 ＋ 0. 25,2. 17 ＋ 0. 33,0. 52 ＋ 0. 21,0. 48 ＋ 0. 23,0. 09 ＋ 0. 03,9. 79 ＋ 0. 65] in Glyycrrhizae radix; [5. 13 ＋ 0. 32,2. 11 ＋ 0. 21,0. 47 ＋ 0. 12,0. 57 ＋0. 22,0. 12 ＋ 0. 02,8. 40 ＋ 0. 75] in honey-fried Glyycrrhizae radix-focal Radix paeoniae alba; [6. 47 ＋ 0. 33,2. 67 ＋ 0. 23,0. 56＋ 0. 23,0. 60 ＋ 0. 23,0. 12 ＋ 0. 01,10. 42 ＋ 0. 78] in honey-fried Glyycrrhizae radix-parched Radix paeoniae alba and [8. 76 ＋0. 35,3. 10 ＋ 0. 43,0. 79 ＋ 0. 32,0. 76 ＋ 0. 34,0. 15 ＋ 0. 05,13. 56 ＋ 0. 99] in honey-fried Glyycrrhizae radix-vigar baked Radix paeoniae alba. Conclusion： Different combination of herbs changed the physical and chemical nature of the single herb,and thus changed its active components. The crucial point in Chinese medicine decoction lies in different components and their proportion of active components. These could affect the curative effect of decoction. It is of great significance to discriminate different active constitutes in drug pairs as it has distinct clinical effect.