胃癌中人端粒保护蛋白1的表达及其与幽门螺杆菌感染的相关性研究

Expression of human protection of telomere 1 and its relationship with Helicobacter pylori infection in gastric carcinoma

目的 探讨胃癌中人端粒保护蛋白1（hPOT1）基因的表达及其与幽门螺杆菌（HP）感染的关系.方法 应用SP免疫组织化学、原位杂交法检测hPOT1蛋白、hPOT1 mRNA在53例胃癌标本（观察组）中的表达,同时以20例正常胃黏膜组织作为对照.应用Warthin-Starry银染法检测53例胃癌组织中HP感染情况.结果 观察组中hPOT1蛋白的阳性表达率为84.91％（45/53）,高于对照组的30.00％（6/20） （P＜0.01）;观察组中hPOT1 mRNA的阳性表达率为58.49％（31/53）,高于对照组的10.00％（2/20）（P＜ 0.05）.胃癌组织中hPOT1蛋白与hPOT1 mRNA共同阳性表达率为56.60％（30/53）,两者在胃癌中的表达呈正相关（r=0.394,P＜0.01）.53例胃癌组织中HP感染阳性28例（52.83％）.HP感染阳性组hPOT1蛋白阳性表达率为96.43％（27/28）,高于HP感染阴性组的72.00％（18/25）（P＜0.05）.HP感染阳性组hPOT1 mRNA阳性表达率为85.75％（24/28）,高于HP感染阴性组的28.00％（7/25）（P＜0.01）.结论 hPOT1可能参与了胃癌的发生,同时检测hPOT1蛋白与hPOT1 mRNA的表达水平有助于胃癌的诊断.胃癌组织中HP感染引起hPOT1异常表达可能是导致胃癌发生的机制之一.

Objective To determine the expression of human protection of telomeres 1 （hPOT1） and its relationship with Helicobacter pylori （HP） infection in gastric carcinoma.Methods The expressions of hPOT1 protein and hPOT mRNA were detected in 53 gastric carcinoma specimens （observed group） and 20 normal gastric mucosa tissues （control group） by SP immunohistochemical method and in situ hybridization （ISH）, respectively.HP infection was examined by Warthin-Starry method in observed group.Results The positive expression rate of hPOT1 protein was 84.91% （45/53） in observed group, higher than that in control group [30.00 % （6/20）] （P 〈 0.01）.The positive expression rate of hPOT1 mRNA was 58.49 % （31/53） in observed group, higher than that in control group [10.00 % （2/20）] （P 〈 0.05）.The positive co-expression rate of hPOT1 protein and mRNA was 56.60 % （30/53）, both had positive relationship in gastric carcinoma （r =0.394, P 〈 0.05）.The rate of HP infection in 53 cases of gastric carcinoma was 52.83 % （28/53）.The positive expression rates of hPOTI protein and mRNA in observed group with HP infection were significantly higher than those in observed group without HP infection[protein： 96.43 % （27/28） vs 72.00 % （18/25）, P 〈0.05;mRNA： 85.75 % （24/28） vs 28.00 % （7/25）, P 〈 0.01].Conclusions hPOT1 may be associated with occurrence of gastric carcinoma.Combined detection of hPOT1 protein and mRNA can be used for the diagnosis of gastric carcinoma.HP infection may be associated with abnormal expression of hPOT1 in occurrence of gastric carcinoma.