胃癌患者血清多种细胞因子联合检测的临床意义

Clinical significance of combined detection of multiple cytokines in serum of patients with gastric cancer

目的 探讨胃癌患者血清中各细胞因子表达及其与临床特征间的关系.方法 运用高通量液相芯片技术对46例胃癌术前患者（胃癌组）和30名健康人（对照组）血清中ITAC、Fractalkine、白细胞介素8（IL-8）、IL-17A、IL-7、肿瘤坏死因子α（TNF-α）表达水平进行检测,以中位数（四分位数间距）表示.探讨各种细胞因子之间的相关性,并分析各个细胞因子与胃癌患者临床特征之间的关系.结果 胃癌组与健康对照组比较,血清中ITAC、Fractalkine、IL-8、IL-17A、IL-7、TNF-α表达水平升高,两组间各指标中位数（四分位数间距）比较差异均具有统计学意义[22.26 pg/ml（32.83 pg/ml）比11.95 pg/ml（9.99 pg/ml） ,P=0.001;62.21 pg/ml（82.23 pg/ml）比26.47 pg/ml（50.87 pg/ml）,P=0.050;4.50 pg/ml（10.38 pg/ml）比2.06 pg/ml（3.17 pg/ml） ,P=0.002;0.83 pg/ml（2.01 pg/ml）比0.21 pg/ml （0.85 pg/ml）,P=0.013;3.46 pg/ml（1.90 pg/ml）比2.11 pg/ml（1.48 pg/ml）,P=0.001;1.21 pg/ml（1.13 pg/ml）比0.79 pg/ml（0.37 pg/ml）,P＜0.001].各细胞因子表达之间具有相关性（均P＜ 0.05）.胃癌有无淋巴结转移患者血清中各细胞因子表达水平差异无统计学意义（均P＞ 0.05）.结论 ITAC、Fractalkine、IL-8、IL-17A、IL-7、TNF-α的高表达可能与胃癌的发生、发展有关.血清中高水平IL-8可能是预后不良的指标.各细胞因子之间的相互作用、相互影响与肿瘤的发生有着一定的关系.

Objective To detect the expression levels of serum ITAC, Fractalkine, IL-8, IL-17A, IL-7 and TNF-α in patients with gastric cancer, and to explore correlation among them, as well as their association with different clinical characteristics.Methods The levels of the 6 kinds of cytokines in serum of 46 gastric cancer patients （gastric cancer group） and 30 healthy people （healthy control group） were detected.Results Compared with those in healthy control group, the levels of serum ITAC, Fractalkine, IL-8, IL-17A, IL-7 and TNF-α in gastric cancer group were significantly increased [22.26 （32.83） pg/ml vs 11.95 （9.99） pg/ml, P =0.001;62.21 （82.23） pg/ml vs 26.47 （50.87） pg/ml, P =0.050;4.50 （10.38） pg/ml vs 2.06 （3.17） pg/ml, P =0.002;0.83 （2.01） pg/ml vs 0.21 （0.85） pg/ml, P=0.013;3.46 （1.90） pg/ml vs 2.11 （1.48） pg/ml, P=0.001;1.21 （1.13） pg/ml vs 0.79 （0.37） pg/ml, P 〈 0.001].There were correlations between cytokines （all P 〈 0.05）.The level of serum cytokines was no significant difference between gastric cancer patients with lymph node metastasis and those without lymph node metastasis （P 〉 0.05）.Conclusions The high level of serum ITAC, Fractalkine, IL-8, IL-17A, IL-7 or TNF-α may be related to the occurrence and development of gastric cancer.High level of serum IL-8 may be a marker of poor prognosis of gastric cancer, and interaction between the various cytokines also has a certain association with tumorigenesis.