不同刺激量针刺关元穴对寒凝类痛经模型大鼠的影响

Different acupuncture stimulus at“Guanyuan(RN4) ”on dysmenorrheal rats model with cold syndrome

目的观察不同的针刺刺激量施于关元穴对寒凝类痛经模型大鼠子宫组织中收缩素受体(OTR)及肌球蛋白轻链激酶(MLCK)含量的影响。方法将处于动情间期3月龄的SD雌性大鼠32只,随机分为盐水组、寒凝类痛经模型组24只,造模成功后将寒凝类痛经模型组又分为模型组、刺激量A组和刺激量B组,每组8只。除盐水组外,模型组、刺激量A组和刺激量B组均采用全身冷冻法结合苯甲酸雌二醇注射法造模。盐水组和模型组不予针刺,刺激量A组予以粗针、深刺、行手法;刺激量B组予以细针、浅刺、不施手法。采用荧光定量PCR方法检测大鼠子宫组织中缩宫素受体(OTR)的含量。采用ELISA(酶联免疫法)测量大鼠子宫组织中MLCK的含量。结果与盐水组比较,模型组的子宫收缩波个数、波峰峰值、活动度及MLCK水平均升高(P<0.01);与模型组比较,刺激量A组的子宫收缩波个数明显减少(P<0.05)、MLCK含量明显降低(P<0.01),刺激量B组的OTR mRNA相对表达量降低(P<0.05);与刺激量A组比较,刺激量B组收缩波个数增多(P<0.05)、OTR mRNA相对表达量降低(P<0.01)。结论不同刺激量针刺关元穴对寒凝类痛经模型大鼠的效应有所不同,粗针、深刺、行手法的效应较强于细针、浅刺、不行手法的针刺效应,提示针刺的刺激量也是决定针刺疗效的因素之一。

Objective To investigate the analgesia mechanism of different acupuncture stimulus at ＂Guanyuan（ RN4） ＂on dysmenorrheal rats model with cold syndrome by observing the concnet of contraction receptor（ OTR）and myosin light streptokinase（ MLCK） of uterine tissue in rats. Methods The 32 female SD rats at diestrus with 3months aged were randomized into 4 groups,including a saline control group,a cold stagnation of dysmenorrheal model control group,an A stimuli group and a B stimuli group,with 8 rats in each group. Except the saline control group rats given intraperitoneal injection 0. 9% Nacl,in the rest groups rats were treated with the whole body freezing method combined with estradiol benzoate injection mold. Results Except the saline control group and model control group,the acupuncture groups acupunctured the RN4 point for 20 min after modeling,the A stimuli group needled acupoint with thick needle and insert deeper with manipulation; the B stimuli group with fine needle and insert shallowly without manipulation. Observe the OTR by Polymerase Chain Reaction（ PCR） and MLCK by ELISA in uterine of 8 rats in each group. Conclusion Different acupuncture stimulus at RN4 on experimental cold syndrome of dysmenorrheal model rats have different effect,inserting deeper with thick needle and exerting manipulation is better than fine needle and inserting shallowly without manipulation. It indicate that different acupuncture stimulus is one of the vital factors to determine the acupuncture therapeutic efficacy.