HPLC法测定北败酱中绿原酸含量

Determination of chlorogenic acid in ixeris chinensis by HPLC

目的建立HPLC法测定北败酱中绿原酸含量的方法。方法采用色谱柱Diamonsil（5μm,4.6 mm×250 mm）;流动相乙腈-0.4%磷酸溶液（1387）;检测波长327 nm;流速1.0 m L/min;柱温35℃。结果绿原酸在0.04～0.36μg（r=0.999 5）范围内,线性关系良好,加样回收率（n=6）为99.27%（RSD=1.03%）。结论本法简便,准确,重现性好,可作为评价北败酱的质控方法。

Objective To establish a method for determination of chlorogenic acid in Ixeris Chinensis by HPLC.Methods A column of Diamonsil（ 5 μm,4. 6 mm × 250 mm） was adopted,and the mobile phase was the mixture of acetonitrile-0. 4% phosphoric acd and water（ 1387） at a flow rate of 1. 0 m L / min; The wavelength was set at 327 nm.Results There was a good linear relationship in the concentration range of 0. 04 ～ 0. 36 μg（ r = 0. 999 5） of chlorogenic acid; The average recovery（ n = 6） was 99. 27%（ RSD = 1. 03%）. Conclusion The method is simple,accurate,and can be used for the quantitative analysis of Ixeris Chinensis.