摘要
目的:通过建立大鼠心肌梗死模型,探讨麝香保心丸(Shexiang Baoxin Pill,SBP)促进血管新生的效应和相关机制。方法:通过结扎冠脉前降支构建大鼠心肌梗死模型并给予SBP及20-HETE特异性合成抑制剂(HET0016)干预8周,观察心梗后心脏结构和功能、梗死交界区VEGF的变化及循环血中内皮祖细胞(endothelial progenitor cells,EPCs)的数量、20-HETE的变化。结果 :SBP可上调心梗后大鼠循环血中的20-HETE(0.35±0.02vs.0.56±0.04 ng/ml,P<0.05),有助于提高心梗后心脏射血分数[(46.3±4.20%vs.(62.30±3.40)%,P<0.05],增加梗死交界区VEGF(3.50±0.50 vs.9.80+1.00,P<0.05)及循环血中的EPCs的数量(0.16±0.06 vs.0.38±0.09,P<0.05),20-HETE特异性抑制剂可阻断SBP的效应。结论 :SBP可通过20-HETE调控EPCs促进血管新生。
Objective：The present study was to explore whether Shexiang Baoxin Pill（SBP） could promote angiogenesis through 20-HETE mediated-endothelial progenitor cells（EPCs） mobilization in a rat model of myocardial infarct.Methods： Experimental myocardial infarction（MI） model was established in rats via occlusion of the left anterior descending coronary artery. SBP and a specific inhibitor of 20-HETE biosynthesis（HET0016） were administrated for eight weeks after MI surgery. Cardiac function was evaluated by echocardiography. The changes of VEGF in the border area of MI were measured. 20-HETE and EPCs in the circulating blood were detected. Results ：SBP improved the heart ejection fraction [（46.3 ±4.20）% vs.（62.30 ±3.40）%, P〈0.05 ] after MI, increased VEGF（3.50 ±0.50 vs.9.80 ＋1.00, P〈0.05） and EPCs（0.16 ±0.06 vs. 0.38 ±0.09, P〈0.05） in the border area of MI 20-HETE biosynthesis inhibitor can block effect of SBP. Conclusion ： SBP can promote angiogenesis by 20-HETE regulatedEPC smobilization.
出处
《岭南急诊医学杂志》
2016年第1期4-6,24,共4页
Lingnan Journal of Emergency Medicine
基金
国家自然科学基金(81370837
81170647)
中国中西医结合学会-和黄科研基金(2013002)
