督脉电针结合游泳训练对大鼠全横断脊髓损伤后GAP-43和Nogo-A表达的影响

Effects of Du Meridian Electroacupuncture combined with swimming training on the expression of NogoA and GAP-43 aftert acute complete spinal cord injury in rats

目的:通过检测大鼠脊髓损伤后运动功能恢复情况和神经生长相关蛋白-43(GAP-43)、神经轴突生长抑制剂-A(Nogo-A)的表达来探讨督脉电针结合游泳训练干预脊髓损伤的作用机制。方法:选用雌性SD大鼠180只,随机分为正常组、假手术组、模型组、游泳训练组、督脉电针组(督电组)、督脉电针结合游泳训练组(联合组)。用外科手术尖形刀片横切断暴露脊髓的方法致大鼠T9-T10段脊髓全横断模型。术后各时间点对各组大鼠进行BBB评分;免疫组化检测各时间点损伤段脊髓GAP-43和Nogo-A的表达;Western blot检测各时间点损伤段脊髓Nogo-A的表达。结果:与模型组相比,联合组和督电组BBB评分显著增加,联合组在术后第14、21、28、35天较督电组BBB评分显著增加,具有显著性差异(P<0.05)。与模型组相比,督电组和联合组GAP-43的表达显著增加,Nogo-A的表达显著减少,具有显著性差异(P<0.05),联合组在术后第14、21、28、35天较电针组GAP-43的表达显著增加,Nogo-A的表达显著减少,具有显著性差异(P<0.05)。结论:(1)督脉电针和游泳训练都能通过促进脊髓损伤大鼠GAP-43的表达和抑制Nogo-A的表达来促进神经的修复。(2)联合治疗对大鼠运动功能的恢复更为有效。

Objective：To investigate the mechanism underling the effects of Du Meridian Electroacupuncture combined with swimming training treatment on spinal cord injury by detecting the motor function recovery and the expression of growth associated protein-43（GAP-43） and neurite outgrowth inhibitor-A（Nogo-A） in the spinal lesion segment.Method：A total of 180 female rats were randomly divided into the normal group, Sham-control group, the operated control group（Op- control group）,swimming training treatment group,Du Meridian electroacupuncture treatment group（DMEA group） and DMEA combined with swimming training treatment group（Combination treatment group）. Acute complete spinal cord contusion model was made at T9-T10 segments with surgical pointed blade cutting off the exposure of the spinal cord. The rats in each group were given BBB score at all time points after the operation. The expression of Nogo- A and GAP- 43 were determined by immunohistochemical method. Furthermore, the expression of Nogo-A was also determined by Western Blot method.Result： The BBB score increased significantly in the DMEA group and combination treatment group compared with the Op-control group. Moreover, the BBB score of the combination treatment group was significantly higher than that of the DMEA group at the days 14,21,28 and 35 post-operation（P〈0.05）. Comparing with the Opcontrol group, the expression of GAP- 43 significantly increased with significantly decreased Nogo- A in the DMEA group and combination treatment group（P〈0.05）. Furthermore, the expression of GAP-43 significantly increased and the expression of Nogo- A significantly decreased in the combination treatment group when compared with the DMEA group at the days 14,21,28 and 35 post-operation.Conclusion：DMEA and swimming training treatment can increase the expression of GAP-43 and decrease the expression of Nogo- A, by which the nerve repair gets promoted. DMEA combined with swimming training treatment is more effective for the motor function recovery.