大黄总蒽醌对大鼠子宫中Bcl-2、Bax表达的影响及可逆性

EFFECTS OF TOTAL ANTHRAQUINONE IN RHEUM ON THE EXPRESSION OF BCL-2 AND BAX IN THE UTERUS OF RATS AND ITS REVERSIBILITY

目的观察长期灌胃大黄总蒽醌对大鼠子宫中Bcl-2、Bax蛋白表达的影响及其可逆性。方法 8周龄SD雌性大鼠40只,随机取10只做为正常对照,其余30只灌胃大黄总蒽醌73mg/kg,连续给药60d。末次给药后24h、停药30d、60d分别处死10只。取子宫,采用免疫组化S-P法检测子宫中Bcl-2、Bax蛋白表达水平。结果给药60dBax表达水平显著高于正常对照(P<0.05),Bcl-2表达显著低于正常对照(P<0.05)。停药30dBax表达水平低于给药60d、高于正常对照(P<0.05),Bcl-2表达水平高于给药60d、低于正常对照,差异有统计学意义(P<0.05)。停药60dBax表达水平明显低于给药60d、停药30d(P<0.05),与正常对照比较差异无统计学意义(P>0.05);Bcl-2表达水平明显高于给药60d、停药30d(P<0.05),并且低于正常对照组(P<0.05)。结论长期应用大黄总蒽醌可降低成年雌性大鼠子宫内膜中Bcl-2蛋白表达,提高Bax蛋白表达,这种影响是可逆的。

Objective To observe the effect and its reversibility of Bax and Bcl-2 protein expression in the uterus of rats with administrating total Anthraquinone in Rheum.Methods Forty 8-week-old SD female rats were selected,10 rats were randomly taken as normal control,the other 30 with administrating total Anthraquinone in Rheum73mg/kg for 60 d,at the end of 24 h after administration,stop drug 30 days and60 days were sacrificed at 10 rats.Then got the uterus,and detected the expression level of Bcl-2 and Bax by immunohistochemical S-P method.Results The levels of Bax expression in the 60 days of administration were significantly higher than those in normal controls（P〈0.05）,and the expression of Bcl-2 was significantly lower than that of normal controls（P〈0.05）.Bax expression level at 30 days after stopping drug was lower than administered 60 d,but higher than the NC（P〈0.05）;Bcl-2 expression level was higher than administered 60 d,but lower than the NC（P〈0.05）.The levels of Bax expression in the 60 days of drug withdrawal were significantly lower than those administered for 60 days（P〈0.05）,but there was no significant difference in the expression of the normal controls（P〉0.05）;the expression level of Bcl-2 was significantly higher than that of administration for 60 days and differences were statistically significant with NC（P〈0.05）.The expression of Bax and Bcl-2 was significantly different between the 30 days and the 60 days of the drug withdrawal（P〈0.05）.Conclusion The long-term use of Rhubarb Anthraquinone can cause the protein expression of Bcl-2 significantly decreased,Bax protein expression is significantly increased in the adult female rat uterus,its toxicity is reversible.