大气压化学萃取电离质谱法用于吡啶类化合物的快速检测

Rapid Detection of Pyridine Compounds by Extractive Atmospheric Pressure Chemical Ionization Mass Spectrometry

利用大气压化学萃取电离源质谱技术(EAPCI-MS)对吡啶类化合物的电离行为特征进行研究。实验显示,EAPCI-MS技术在常温常压条件下,无需样品预处理和任何辅助化学试剂,在质谱图中能同时检测到吡啶类化合物的质子化分子离子峰[M+H]+和分子离子峰M+·,并具有类似的二级碎裂机理。研究结果表明,EAPCI-MS技术具有不同于传统电离源质谱的裂解方式,同时兼具传统电喷雾电离(ESI)和大气压化学电离(APCI)的特征电离方式和行为,极大地提高了化学检测的选择性,增强了质谱分析的定性能力。该研究为吡啶类化合物的检测和鉴定提供了一种新方法,对吡啶类化合物的快速检测具有重要的应用价值和意义。

The fragmentation mechanisms of pyridine compounds in extractive atmospheric pressure chemical ionization mass spectrometry（EAPCI-MS） analysis were investigated.The experiments showed that under the ambient conditions,the protonated molecular ion peak [M ＋ H]^＋and molecular ion peak M^＋ ·could be simultaneously observed using the EAPCI-MS,and similar fragmentation rules can be obtained for their collision induced dissociation（CID）,without any sample pretreatment and supporting reagent.It is obvious that,compared with that of the traditional ion sources,the chemical selectivity and qualitative analysis of EAPCI source are significantly improved for the rapid detection of pyridine compounds,in which the EAPCI source has both the fragment characteristics of traditional ion sources of ESI and APCI.This study provided a novel method for the qualitative detection of pyridine compounds.