超高效液相色谱-串联质谱法同时测定纺织品中4种苯并三唑类紫外稳定剂

Simultaneous Detection of Four Benzotriazole UV Stabilizers in Textiles by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry

建立了快速同时测定纺织品中UV-350,UV-320,UV-328和UV-327 4种苯并三唑类紫外稳定剂（BUVSs）的超声提取/超高效液相色谱-串联质谱（USE-UPLC-MS/MS）分析方法。样品经正己烷饱和的乙腈超声波提取后,采用Eclipse Plus C18柱（2.1 mm×50 mm,1.8μm）分离,甲醇-0.01 mol/L甲酸铵水溶液为流动相梯度洗脱,电喷雾离子源电离,正离子多反应监测模式进行定性和定量分析,外标法定量。结果表明,4种BUVSs的线性相关系数（r^2）均大于0.99,检出限为0.006 3-0.024 mg/kg,定量下限为0.021-0.081 mg/kg。加标回收率为80.0%-102.9%,相对标准偏差（RSD）为1.2%-9.9%。将该方法应用于纺织品中BUVSs的分析测定,结果令人满意。

A method was developed for the simultaneous determination of four benzotriazole UV stabilizers（BUVSs） including UV-350,UV-320,UV-328 and UV-327 in textiles by ultra-high performance liquid chromatography-tandem mass spectrometry（UPLC-MS / MS） combined with ultrasonic extraction（USE）.The samples were extracted with acetonitrile saturated with n-hexane under ultrasonic assistance.The separation was performed on an Eclipse Plus C18column（2.1 mm × 50 mm,1.8 μm） using 0.01 mol / L ammonium formate-methanol as mobile phase.Identification and quantification were achieved by UPLC-MS / MS coupled with electrospray ionization（ESI） source in positive ion mode and multiple reactions monitoring（MRM） mode,and the concentration of each analyte was calibrated by the external standard method.The result indicated that the calibration curves of four BUVSs showed good linear relationships between peak areas and certain concentration ranges with correlation coefficients（r^2） greater than 0.99.The limits of detection for UV-350,UV-320,UV-328,UV-327 were 0.023,0.024,0.006 3,0.021 mg / kg,respectively.The limits of quantitation were 0.078,0.081,0.021,0.072 mg / kg,respectively.The recoveries at three spiked concentration levels were in the range of 80.0%-102.9% with relative standard deviations（RSDs）of 1.2%-9.9%.The developed method was successfully applied in the determination of BUVSs in textiles.