橙皮苷减轻大鼠心肌缺血/再灌注损伤所致的炎症反应

Hesperidin Reduces Inflammatory Response During Rat Myocardial Ischemia/Reperfusion Injury

目的:探讨橙皮苷能否通过其抗炎作用减轻大鼠心肌缺血/再灌注损伤。方法:24只雄性SD大鼠随机分为假手术组、缺血/再灌注组(I/R组)及橙皮苷组,每组8只。结扎左冠状动脉前降支30min再灌注4h建立大鼠心肌缺血再灌注损伤模型。HE染色法光镜下观察大鼠心肌组织形态改变,量子点免疫荧光法荧光镜下观察心肌组织高迁移率族蛋白1(HMGB1)的表达,全自动生化分析仪测定血清中肌酸激酶(CK)和乳酸脱氢酶(LDH)的水平,ELISA法和Westen blot法分别检测心肌组织中白介素6(IL-6)、肿瘤坏死因子α(TNF-α)的水平及HMGB1的表达。结果:与I/R组相比,橙皮苷能减轻心肌组织损伤程度,降低心肌酶CK及LDH的水平,降低心肌组织炎症指标TNF-α和IL-6的含量及HMGB1的表达(P<0.05)。结论:橙皮苷能减轻心肌缺血/再灌注损伤,其机制与其抑制HMGB1的表达相关。

Objective：To investigate whether hesperidin could protect against rat myocardial ischemia/reperfusion（I/R）injury by inhibiting inflammatory response.Methods：Twenty-four male SD rats were randomly divided into sham operated group,ischemia/reperfusion（I/R）group and hesperidin group with eight rats in each.Myocardial I/R injury model was established by ligaturing the left anterior descending artery for 30 min followed reperfusion for 4h.HE stain was used to observe myocardium status under light microscope,quantum dots-tagged fluorescence technology was used to observe the expression of high mobility group box 1（HMGB1）in myocardium under fluorescence microscope,cardiac injury biomarkers（creatine kinase,CK and lactate dehydrogenase,LDH）were measured by automatic biochemistry analyzer,the levels of tumor necrosis factor alpha（TNF-α）and interleukin-6（IL-6）and the expression of HMGB1 were measured by ELISA and Western blot,respectively.Results：Compared with I/R group,the degree of myocardium damage and the levels of cardiac injury biomarkers（CK and LDH）in serum significantly decreased in hesperidin group（P〈0.05）.Hesperidin also significantly decreased the levels ofTNF-α,IL-6 and HMGB1 in myocardium when compared with I/R group（P 0.05）.Conclusion：Hesperidin attenuates myocardial I/R injury by inhibiting HMGB1 expression.