基于代谢组学研究橙黄决明素对高脂血症大鼠的调脂作用

Metabolomics-based Study of the Lipid Regulating Effect of Aurantio-obtusin on Hyperlipidemia Rats

本文运用代谢组学的研究方法,考察橙黄决明素对高脂血症大鼠血液内源性代谢物的影响,并寻找相关生物标志物。采用高脂饲料喂养的方法建立高脂血症大鼠模型,给予橙黄决明素灌胃治疗。利用气相色谱-质谱(GC-MS)联用技术分析高脂血症大鼠血浆中的代谢物,并使用主成分分析、偏最小二乘-判别分析和随机森林算法,研究大鼠样本在造模前后及橙黄决明素治疗后血浆内源性代谢物的变化情况,结果得到丙氨酸、甘氨酸、L-缬氨酸、异亮氨酸、富马酸、丝氨酸、1,5-脱水山梨醇、亚油酸、硬脂酸和胆固醇10种潜在的生物标志物。研究表明橙黄决明素主要通过影响机体氨基酸及脂肪酸的代谢,从而产生较理想的调脂作用。

The influences of aurantio-obtusin on endogenous metabolites in blood of hyperlipemia rats were investigated by metabonomics method in order to find the related biomarkers.The rat model was established by hyperlipidemia rats that were raised by high fat diet and then treated with intragastric administration of aurantio-obtusin.Metabolites in plasma of hyperlipidemia rats were accurately analyzed by gas chromatography-mass spectrometry.In order to find out the potential biomarkers,principal component analysis,partial least squares discriminant analysis and random forest algorithm were used to study the changes of endogenous metabolite profiles in rats before modeling after modeling and after drug treatments.Ten potential biomarkers were obtained as the final achievement：alanine,glycine,valine,isoleucine,fumaric acid,serine,1,5-anhydro-D-ghlcitol,linoleic acid,stearic acid and cholesterol.This investigation demonstrates that aurantio has a good lipid regulating effect mainly by affecting the body amino acid and fatty acid metabolism.