La(OH)_3纳米片修饰玻碳电极对鸟嘌呤和腺嘌呤的电催化氧化

Electrocatalytic Oxidation of Guanine and Adenine Based on the La(OH)_3 Nano-plates Modified Glassy Carbon Electrode

以La（OH）3纳米片为修饰剂,制备了基于La（OH）3纳米片修饰玻碳电极（LNP/GCE）。采用循环伏安（CV）法研究了鸟嘌呤（G）和腺嘌呤（A）在该修饰电极上的电化学行为。实验结果表明,在HAc-NaAc缓冲溶液中,该修饰电极对G和A都表现出了良好的电催化性能。在最佳条件下,用差分脉冲伏安（DPV）法对G和A进行了测定,其氧化峰电流与G和A的浓度在0.1-10μmol/L范围内呈良好的线性关系,检测限（S/N=3）分别为0.01μmol/L和0.03μmol/L。将该修饰电极用于DNA中A和G的同时测定,获得较好结果。

A novel and reliable electrochemical sensor based on the Lanthanum hydroxide nano-plates modified glassy carbon electrode（LNP/GCE）was developed for sensitive determination of guanine（G）and adenine（A）.Electrochemical behavior of LNP/GCE was investigated by using cyclic voltammetry（CV）and electrochemical impedance spectroscopy（EIS）.The results indicated that both G and A showed the increase of the oxidation peak currents with the negative shift of the oxidation peak potentials.Well separated voltammetric peaks were obtained at the modified electrode for G and A.Differential pulce voltammetry（DPV）was used for simultaneous determination of G and A under the optimal conditions.The oxidation peaks current of both G and A showed linear dependence on their concentrations in the range of 0.1-10μmol/L,with the detection limit（S/N=3）of 0.01μmol/L and0.03μmol/L,respectively.Furthermore,the proposed method was successfully applied for the measurement of G and A in herring sperm DNA samples with satisfactory results.