基于胆固醇改性壳聚糖材料的蛋白吸附及细胞相容性评价

Protein adsorption and cell compatibility evaluation based on cholesterol modified chitoan

目的:提高壳聚糖作为生物材料对细胞的主动响应能力.方法:利用胆固醇对壳聚糖进行仿生修饰.通过QCM-D技术考察了壳聚糖改性前后对于白蛋白吸附行为的变化,AFM技术考察了壳聚糖及其衍生物吸附白蛋白前后的膜表面形貌变化;成纤维细胞3T3评价了细胞在不同材料表面的增殖及形态.结果:经胆固醇修饰后的壳聚糖膜表面蛋白吸附能力有显著提升,改性程度越高,白蛋白的吸附能力越强.吸附蛋白前胆固醇-g-壳聚糖膜表面粗糙度随着改性程度增大而升高,而后下降.吸附蛋白后,膜表面粗糙度反而下降,但高改性程度的膜表面却升高.结论:细胞实验结果表明,胆固醇改性后的壳聚糖衍生物具有较好的细胞相容性,且改性后的胆固醇-g-壳聚糖材料在不同程度上具有促进细胞黏附与增殖的能力.

Aim： In order to improve the ability of chitosan as a function biomaterials and the active response ability to cells. Methods： cholesterol modified chitosan conjugate with succinyl linkages was synthesized. Effects of chitosan （CS） and cholesterol-g-chitosan （CHCS） on adsorption of albumin behavior changes were characterized using QCM-D technology, the film surface morphology changes before and after adsorption of albumin was characterized using AFM technique. The proliferation and morphology of 3T3 cells on CS and CHCS materials were assessed too. Results： The protein adsorption capability of CHCS membrane surface was increased obviously with the graft rate of cholesterol increasing. The surface roughness of the CHCS membrane increased along with the modified degree increasing before protein ad- sorption, but then decreased when modified degree was high. However, the surface roughness of CHCS membrane decreased after protein absorption, but then increased when modified degree was high. Conclusion： The cell experiment result show that the CHCS materials have good cellular compatibility, and have the ability to promote cell adhesion and proliferation in different degrees compare to pure CS materials