摘要
目的研究系统性红斑狼疮(SLE)小鼠(MRL/lpr)经青蒿-鳖甲药对治疗后血清代谢物的变化,探讨SLE发病及药物作用机制。方法采集对照组、SLE模型组和青蒿-鳖甲药对治疗组(低、高剂量组)小鼠的血清样本,应用高效液相色谱-四级杆-飞行时间质谱联用系统(HPLC-Q-TOF/MS),对样本进行代谢图谱分析。应用Mass Hunter和MPP软件处理数据,数据模式识别采用有监督的偏最小二乘法-判别分析(PLS-DA)。结果代谢组学分析发现,SLE模型小鼠存在较明显的脂代谢紊乱。与模型组相比,药物组可以改善狼疮鼠体内脂质代谢异常,影响SLE疾病血栓的发生发展;并且可以改善SLE小鼠急性期的炎症反应,减少其病理损害。结论青蒿-鳖甲药对通过调节SLE小鼠的脂质代谢及炎症反应对其发挥一定的治疗作用。
Aims To study the changes of serum me-tabolites in systemic lupus erythematosus ( SLE ) mice ( MRL/lpr) by treatment of Qinghao-Biejia and to ex-plore the pathogenesis of SLE and mechanism of drug action. Methods The serum samples of control group, SLE model group and Qinghao-Biejia treatment group ( low and high dose ) were collected, the metabolic profile of samples was analyzed by high performance liquid chromatography-quadrupole-time of flight mass spectrometry system ( HPLC-Q-TOF/MS ) . Software of Mass Hunter and Mass Profiler Professional ( MPP ) were used to process the data. A supervised mode of partial least squares-discriminant analysis ( PLS-DA ) was applied to recognize the data pattern. Results There were obvious disorders of lipid metabolism in SLE model. Compared with control group, Qinghao-Biejia treatment group improved lipid metabolism, af-fected the thrombosis development of SLE; and Qing-hao-Biejia treatment group reduced the pathological damage by improving inflammatory acute phase of SLE in mice. Conclusion Qinghao-Biejia treatment plays a therapeutic role in repairing the imbalance by multidi-mensional metabolic pathways in SLE mice.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2016年第5期727-732,共6页
Chinese Pharmacological Bulletin
基金
国家公益性行业科研专项(No 2015468001)
国家自然科学基金资助项目(No 81373633
81273680)
浙江省重大科技专项(No 2014C03062-1)
浙江省科技厅公益性应用研究计划项目(No 2013C37100)
关键词
青蒿-鳖甲药对
系统性红斑狼疮
小鼠
血清
代谢组学
液相色谱-四级杆-飞行时间质谱
traditional Chinese herb pair
QinghaoBiejia
systemic lupus erythematosus
mice
serum
metabonomics
high performance liquid chromatography coupled with Q-TOF / MS