北京地区草莓灰霉病菌的转座子及其分布频率

Presence and frequency distribution of transposable elements in Botrytis cinerea from strawberry in Beijing

为了解北京地区草莓灰霉病菌的转座子类型及其分布,本研究用转座子Boty和Flipper的特异性引物对北京地区2012-2013年从12个草莓园采集和分离的60株草莓灰霉病菌进行PCR扩增。结果表明,北京地区草莓灰霉病菌群体中共存在3种转座子类型:transposa型、Boty型和Flipper型。其中,以transposa型菌株最多,占供试菌株的63.3%,Boty型菌株占供试菌株的28.3%,Flipper型菌株最少,仅占8.4%,未检测到vacuma型菌株。选取属于不同转座子类型的18株菌株测定其对草莓叶片的致病力,结果显示Boty型菌株所致病斑的平均直径显著大于Flipper型。草莓灰霉病菌转座子类型与致病力的关系有待进一步研究。转座子类型的检测为进一步研究灰葡萄孢的遗传多样性及遗传变异提供了基础。

This study was conducted primarily to investigate the presence and frequency distribution of the transposable elements Boty and Flipper in populations of Botrytis cinereafrom12 strawberry gardens in Beijing.A total of 60 isolates were collected from diseased strawberry fruits during 2012-2013,and the presence of transposable elements was analyzed by specific PCR.The results showed that there were three types（transposa,Boty and Flipper）of transposable elements.The transposatype isolates carrying both elements were predominant with frequency of 63.3%,and both Boty type and Flipper type isolates were present with low frequencies of 28.3% and8.4%,respectively,while vacuma type isolate was not detected in this study.The pathogenicity analysis of 18 isolates selected from three types demonstrated that Boty type isolates showed the highest pathogenicity to strawberry leaves,with the larger lesion size than Flipper type and transposa type isolates.The relationship between transposon types and pathogenicity of B.cinerea is needed to study.Detection of transposable elements provides the bases for further study of genetic diversity and variation in B.cinerea.