侵染冬瓜的病毒病原种类鉴定

Identification of pathogenic viruses infecting wax gourd

本研究在我国主要冬瓜产区采集具有典型病毒病症状的病叶材料105份,根据葫芦科作物上常见的5种病毒病原的CP基因设计特异性引物,对105份待检冬瓜材料进行RT-PCR检测。检测结果表明:5对特异引物可分别在105份待检材料的95份中检测到小西葫芦黄花叶病毒(Zucchini yellow mosaic virus,ZYMV)、西瓜花叶病毒(Watermelon mosaic virus,WMV)、黄瓜花叶病毒(Cucumber mosaic virus,CMV)、番木瓜环斑病毒(Papaya ringspot virus,PRSV)4种病毒,未检测到南瓜花叶病毒(Squash mosaic virus,SqMV);并且发现不同的冬瓜主产区致病的病毒种类有较大差异;同时还发现,在这些待检样品中4种病毒复合侵染现象较普遍,其中以PRSV与WMV组合最常见,占复合侵染现象的31.25%;未发现有4种病毒复合侵染。

According to the CP genes of Zucchini yellow mosaic virus（ZYMV）,Watermelon mosaic virus（WMV）,Cucumber mosaic virus（CMV）,Papaya ringspot virus（PRSV）and Squash mosaic virus,（SqMV）,five pairs of specific primers were designed for RT-PCR.A total of 105 diseased wax gourd leaf samples with typical symptoms collected from the main producing area were amplified with the specific primers.ZYMV,WMV,CMV and PRSV were detected in the 95 diseased samples,and all the tested samples showed negative with SqMV.There were different types of pathogenic viruses in different main producing areas.Complex infection is very common,and 31.25% of them is mixed infection of PRSV and WMV,which is the most common one.No infection was found by all four viruses.